Soluble apyrases release ADP during ATP hydrolysis

被引：54

作者：

Chen, W ^{[1
]}

Guidotti, G ^{[1
]}

机构：

[1] Harvard Univ, Dept Mol & Cellular Biol, Cambridge, MA 02138 USA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2001年 / 282卷 / 01期

关键词：

CD39; E-NTPDase; apyrase; ADP; release; binding site; mechanism;

D O I：

10.1006/bbrc.2001.4555

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A soluble form of CD39 was expressed and purified from High-Five insect cells. The soluble CD39 is a monomer with a molecular weight of 54,000. The k(cat) and K-m of the purified soluble CD39 were 4.6 s(-1) and 12 muM for ATP and 1.3 s(-1) and 7 muM for ADP as substrates, respectively. One nucleotide binding site was detected on the monomer only in the presence of Ca2+. In contrast to the membrane bound CD39, soluble CD39 released ADP as an intermediate during ATP hydrolysis, as did the soluble potato apyrase, (C) 2001 Academic Press.

引用

页码：90 / 95

页数：6

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