The Escherichia coli OxyS regulatory RNA represses fhlA translation by blocking ribosome binding

被引：178

作者：

Altuvia, S

Zhang, AX

Argaman, L

Tiwari, A

Storz, G ^{[1
]}

机构：

[1] NICHHD, Cell Biol & Metab Branch, NIH, Bethesda, MD 20892 USA

[2] Hebrew Univ Jerusalem, Hadassah Med Sch, Dept Mol Genet & Biotechnol, IL-91120 Jerusalem, Israel

来源：

EMBO JOURNAL | 1998年 / 17卷 / 20期

关键词：

antisense regulation; oxidative stress; OxyS RNA; ribosome binding; translation;

D O I：

10.1093/emboj/17.20.6069

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

OxyS is a small untranslated RNA which is induced in response to oxidative stress in Escherichia coli. This novel RNA acts as a global regulator to activate or repress the expression of as many as 40 genes, including the fhlA-encoded transcriptional activator and the rpoS-encoded sigma(S) subunit of RNA polymerase. Deletion analysis of OxyS showed that different domains of the small RNA are required for the regulation of fhlA and rpoS, We examined the mechanism of OxyS repression of fhlA and found that the OxyS RNA inhibits fhlA translation by pairing with a short sequence overlapping the Shine-Dalgarno sequence, thereby blocking ribosome binding/translation.

引用

页码：6069 / 6075

页数：7

共 27 条

[1] ALTERNATIVE MESSENGER-RNA STRUCTURES OF THE CIII-GENE OF BACTERIOPHAGE-LAMBDA DETERMINE THE RATE OF ITS TRANSLATION INITIATION [J].