Site-specific labeling of proteins with small molecules in live cells

被引：242

作者：

Chen, I ^{[1
]}

Ting, AY ^{[1
]}

机构：

[1] MIT, Dept Chem, Cambridge, MA 02139 USA

来源：

CURRENT OPINION IN BIOTECHNOLOGY | 2005年 / 16卷 / 01期

关键词：

D O I：

10.1016/j.copbio.2004.12.003

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The principal bottleneck for the utilization of small-molecule probes in live cells is the shortage of methodologies for targeting them with very high specificity to biological molecules or compartments of interest. Recently developed methods for labeling proteins with small-molecule probes in cells employ special protein or peptide handles that recruit small-molecule ligands, harness enzymes to catalyze small-molecule conjugation or hijack the cell's protein translation machinery.

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页码：35 / 40

页数：6

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