DC ablation in mice: promises, pitfalls, and challenges

被引:117
作者
Bennett, Clare L. [1 ]
Clausen, Bjoern E. [2 ]
机构
[1] UCL, Royal Free Hosp, Dept Haematol, London NW3 2PF, England
[2] Univ Amsterdam, Acad Med Ctr, Dept Cell Biol & Histol, NL-1105 AZ Amsterdam, Netherlands
基金
英国惠康基金;
关键词
D O I
10.1016/j.it.2007.08.011
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Dendritic cells (DC) play pivotal roles in orchestrating immunity and tolerance, and, as such, they are key targets for immunotherapy. Exploiting their function depends on a precise understanding of the part that different DC subsets play in vivo, but attempts to identify definitive functions have been limited by problems depleting individual DC populations in mice. Inducible cell ablation via transgenic expression of a high-aff inity diphtheria toxin receptor (DTR) is a new and powerful approach to DC research. Here, we discuss the impact of CD11c-DTR and Langerin-DTR mice on DC immunobiology, and we highlight the problems to be aware of when interpreting data from these models. The challenge now will be to refine transgenic strategies so that other DC subsets can be inducibly depleted in vivo.
引用
收藏
页码:525 / 531
页数:7
相关论文
共 60 条
[1]   Migratory dendritic cells transfer antigen to a lymph node-resident dendritic cell population for efficient CTL priming [J].
Allan, Rhys S. ;
Waithman, Jason ;
Bedoui, Sammy ;
Jones, Claerwen M. ;
Villadangos, Jose A. ;
Zhan, Yifan ;
Lew, Andrew M. ;
Shortman, Ken ;
Heath, William R. ;
Carbone, Francis R. .
IMMUNITY, 2006, 25 (01) :153-162
[2]   Dendritic cells and the control of immunity [J].
Banchereau, J ;
Steinman, RM .
NATURE, 1998, 392 (6673) :245-252
[3]   Inducible ablation of mouse Langerhans cells diminishes but fails to abrogate contact hypersensitivity [J].
Bennett, CL ;
van Rijn, E ;
Jung, S ;
Inaba, K ;
Steinman, RM ;
Kapsenberg, ML ;
Clausen, BE .
JOURNAL OF CELL BIOLOGY, 2005, 169 (04) :569-576
[4]  
BENNETT CL, IN PRESS J IMMUNOL
[5]   Distinct roles of dendritic cells and B cells in Va14Ja18 natural T cell activation in vivo [J].
Bezbradica, JS ;
Stanic, AK ;
Matsuki, N ;
Bour-Jordan, H ;
Bluestone, JA ;
Thomas, JW ;
Unutmaz, D ;
Van Kaer, L ;
Joyce, S .
JOURNAL OF IMMUNOLOGY, 2005, 174 (08) :4696-4705
[6]   A role for endogenous transforming growth factor beta 1 in Langerhans cell biology: The skin of transforming growth factor beta 1 null mice is devoid of epidermal Langerhans cells [J].
Borkowski, TA ;
Letterio, JJ ;
Farr, AG ;
Udey, MC .
JOURNAL OF EXPERIMENTAL MEDICINE, 1996, 184 (06) :2417-2422
[7]   Targeted expression of major histocompatibility complex (MHC) class II molecules demonstrates that dendritic cells can induce negative but not positive selection of thymocytes in vivo [J].
Brocker, T ;
Riedinger, M ;
Karjalainen, K .
JOURNAL OF EXPERIMENTAL MEDICINE, 1997, 185 (03) :541-550
[8]   A Cre-inducible diphtheria toxin receptor mediates cell lineage ablation after toxin administration [J].
Buch, T ;
Heppner, FL ;
Tertilt, C ;
Heinen, TJAJ ;
Kremer, M ;
Wunderlich, FT ;
Jung, S ;
Waisman, A .
NATURE METHODS, 2005, 2 (06) :419-426
[9]   Conditional macrophage ablation demonstrates that resident macrophages initiate acute peritoneal inflammation [J].
Cailhier, JF ;
Partolina, M ;
Vuthoori, S ;
Wu, SJ ;
Ko, K ;
Watson, S ;
Savill, J ;
Hughes, J ;
Lang, RA .
JOURNAL OF IMMUNOLOGY, 2005, 174 (04) :2336-2342
[10]   Production of monoclonal antibodies that recognize the extracellular domain of mouse Langerin/CD207 [J].
Cheong, Cheolho ;
Idoyaga, Juliana ;
Do, Yoonkyung ;
Pack, Maggi ;
Park, Sung Ho ;
Lee, Haekyung ;
Kang, Young-Sun ;
Choi, Jae-Hoon ;
Kim, Jae Y. ;
Bonito, Anthony ;
Inaba, Kayo ;
Yamazaki, Sayuri ;
Steinman, Ralph M. ;
Park, Chae Gyu .
JOURNAL OF IMMUNOLOGICAL METHODS, 2007, 324 (1-2) :48-62