DC ablation in mice: promises, pitfalls, and challenges
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作者:
Bennett, Clare L.
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UCL, Royal Free Hosp, Dept Haematol, London NW3 2PF, EnglandUCL, Royal Free Hosp, Dept Haematol, London NW3 2PF, England
Bennett, Clare L.
[1
]
Clausen, Bjoern E.
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Univ Amsterdam, Acad Med Ctr, Dept Cell Biol & Histol, NL-1105 AZ Amsterdam, NetherlandsUCL, Royal Free Hosp, Dept Haematol, London NW3 2PF, England
Clausen, Bjoern E.
[2
]
机构:
[1] UCL, Royal Free Hosp, Dept Haematol, London NW3 2PF, England
[2] Univ Amsterdam, Acad Med Ctr, Dept Cell Biol & Histol, NL-1105 AZ Amsterdam, Netherlands
Dendritic cells (DC) play pivotal roles in orchestrating immunity and tolerance, and, as such, they are key targets for immunotherapy. Exploiting their function depends on a precise understanding of the part that different DC subsets play in vivo, but attempts to identify definitive functions have been limited by problems depleting individual DC populations in mice. Inducible cell ablation via transgenic expression of a high-aff inity diphtheria toxin receptor (DTR) is a new and powerful approach to DC research. Here, we discuss the impact of CD11c-DTR and Langerin-DTR mice on DC immunobiology, and we highlight the problems to be aware of when interpreting data from these models. The challenge now will be to refine transgenic strategies so that other DC subsets can be inducibly depleted in vivo.