Differential association of cytoplasmic signalling molecules SHP-1, SHP-2, SHIP and phospholipase C-γl with PECAM-1/CD31

被引：88

作者：

Pumphrey, NJ

Taylor, V

Freeman, S

Douglas, MR

Bradfield, PF

Young, SP

Lord, JM

Wakelam, MJO

Bird, IN

Salmon, M

Buckley, CD ^{[1
]}

机构：

[1] Univ Birmingham, Div Immun & Infect, Birmingham B15 2TT, W Midlands, England

[2] John Radcliffe Hosp, Inst Mol Med, Cell Adhes Lab, Oxford OX3 9DU, England

[3] Univ Birmingham, Div Canc Studies, Birmingham B15 2TT, W Midlands, England

[4] Univ Birmingham, MRC, Ctr Immune Regulat, Birmingham B15 2TT, W Midlands, England

[5] Yamanouchi Res Inst, Oxford OX4 4SX, England

来源：

FEBS LETTERS | 1999年 / 450卷 / 1-2期

基金：

英国惠康基金;

关键词：

platelet endothelial cell adhesion molecule 1; surface plasmon resonance; protein-protein interaction; Src homology 2 domain;

D O I：

10.1016/S0014-5793(99)00446-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Recent studies have shown that, in addition to its role as an adhesion receptor, platelet endothelial cell adhesion molecule 1/CD31 becomes phosphorylated on tyrosine residues Y-663 and Y-686 and associates with protein tyrosine phosphatases SHP-1 and SHP-2, In this study, we screened for additional proteins which associate with phosphorylated platelet endothelial cell adhesion molecule 1, using surface plasmon resonance. We found that, besides SHP-1 and SHP-2, platelet endothelial cell adhesion molecule 1 binds the cytoplasmic signalling proteins SHIP and PLC-gamma 1 via their Src homology 2 domains. Using two phosphopeptides, NSDVQpY(663)TEVQV and DTETVpY(686)SEVRK, we demonstrate differential binding of SHP-1, SHP-2, SHIP and PLC-gamma 1. All four cytoplasmic signalling proteins directly associate with cellular platelet endothelial cell adhesion molecule 1, immunoprecipitated from pervanadate-stimulated THP-1 cells, These results suggest that overlapping immunoreceptor tyrosine-based inhibition motif/immunoreceptor tyrosine-based activation motif-like motifs within platelet endothelial cell adhesion molecule 1 mediate differential interactions between the Src homology 2 containing signalling proteins SHP-1, SHP-2, SHIP and PLC-gamma 1. (C) 1999 Federation of European Biochemical Societies.

引用

页码：77 / 83

页数：7

共 36 条

[1] Definition of the sites of interaction between the protein tyrosine phosphatase SHP-1 and CD22 [J].