Potato tubers as a biofactory for recombinant antibodies

被引：97

作者：

Artsaenko, O

Kettig, B

Fiedler, U

Conrad, U

During, K

机构：

[1] Fed Ctr Breeding Res Cultivated Plants, Inst Breeding Methods Vegetables, D-06484 Quedlinburg, Germany

[2] Inst Pflanzengenet & Kulturpflanzenforsch, D-06466 Gatersleben, Germany

来源：

MOLECULAR BREEDING | 1998年 / 4卷 / 04期

关键词：

antibody; endoplasmic reticulum; phytofarming; potato; production; scFv;

D O I：

10.1023/A:1009676832273

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Potato tubers have been successfully used for high-level production of a recombinant single-chain Fv (ScFv) antibody. Ubiquitous high-level expression was achieved under control of the CaMV 35S promoter through retention of the scFv protein in the endoplasmic reticulum. Recombinant antibodies accumulated up to 2% of total soluble tuber protein. After 1.5 years of tuber storage at 4 degrees C still half of the amount of scFv present in freshly harvested tubers was detectable. Its specific activity did not decrease during tuber storage. Recombinant protein could be efficiently purified from crude extracts by affinity chromatography.

引用

页码：313 / 319

页数：7

共 24 条

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