Cloning, renal distribution, and regulation of the rat Na+-HCO3- cotransporter

被引：48

作者：

Burnham, CE

Flagella, M

Wang, ZH

Amlal, H

Shull, GE

Soleimani, M

机构：

[1] Univ Cincinnati, Coll Med, Div Nephrol & Hypertens, Dept Med,Sch Med, Cincinnati, OH 45267 USA

[2] Univ Cincinnati, Sch Med, Dept Mol Genet Biochem & Microbiol, Cincinnati, OH 45267 USA

[3] Vet Affairs Med Ctr, Cincinnati, OH 45220 USA

来源：

AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY | 1998年 / 274卷 / 06期

关键词：

proximal tubule; bicarbonate; acidosis; alkalosis;

D O I：

10.1152/ajprenal.1998.274.6.F1119

中图分类号：

Q4 [生理学];

学科分类号：

071003 ;

摘要：

We recently reported the cloning and expression of a human kidney Na+-HCO3- co-transporter (NBC-1) (C. E. Burnham, H. Amlal, Z. Wang, G. E. Shull, and M. Soleimani. J. Biol. Chem. 272: 19111-19114, 1997). To expedite in vivo experimentation, we now report the cDNA sequence of rat kidney NBC-1. In addition, we describe both the organ and nephron segment distributions and the regulation of NBC-1 mRNA under three models of pH stress: chloride-depletion alkalosis (CDA), metabolic acidosis, and bicarbonate loading. Rat NBC-1 cDNA encodes an open reading frame of 1,035 amino acids, with 96 and 87% identity to human and salamander NBC-1, respectively. Rat NBC-1 mRNA is expressed at high levels in kidney and brain, with lower levels in colon, stomach, and heart. None appears in liver. In the kidney, NBC-1 is expressed mainly in the proximal tubule, with traces found in medullary thick. ascending limb and papilla. HCO3- loading decreased NBC-1 mRNA. levels, which were unchanged either by metabolic acidosis or by CDA.

引用

页码：F1119 / F1126

页数：8

共 28 条

[1] PARALLEL ADAPTATION OF THE RABBIT RENAL CORTICAL SODIUM PROTON ANTIPORTER AND SODIUM-BICARBONATE COTRANSPORTER IN METABOLIC-ACIDOSIS AND ALKALOSIS [J].