Electron spin resonance in studies of membranes and proteins

被引：297

作者：

Borbat, PP

Costa-Filho, AJ

Earle, KA

Moscicki, JK

Freed, JH ^{[1
]}

机构：

[1] Cornell Univ, Baker Lab Chem & Chem Biol, Ithaca, NY 14853 USA

[2] Jagiellonian Univ, Smoluchowski Inst Phys, PL-30059 Krakow, Poland

来源：

SCIENCE | 2001年 / 291卷 / 5502期

关键词：

D O I：

10.1126/science.291.5502.266

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We provide a review of current electron spin resonance (ESR) techniques for studying basic molecular mechanisms in membranes and proteins by using nitroxide spin Labels. tn particular, nitroxide spin label studies with high-field/high-frequency ESR and two-dimensional Fourier transform ESR enable one to accurately determine distances in biomolecules, unravel the details of the complex dynamics in proteins. characterize the dynamic structure of membrane domains, and discriminate between bulk Lipids and boundary lipids that coat transmembrane peptides or proteins; these studies can also provide time resolution to studies of functional dynamics of proteins. We illustrate these capabilities with recent examples.

引用

页码：266 / 269

页数：4

共 45 条

[1] Dynamics and ordering in mixed model membranes of dimyristoylphosphatidylcholine and dimyristoylphosphatidylserine: A 250-GHz electron spin resonance study using cholestane [J].