Variant histone H3.3 marks promoters of transcriptionally active genes during mammalian cell division

被引:134
作者
Chow, CM
Georgiou, A
Szutorisz, H
Silva, AME
Pombo, A
Barahona, I
Dargelos, E
Canzonetta, C
Dillon, N
机构
[1] Univ London Imperial Coll Sci Technol & Med, Fac Med, Gene Regulat & Chromatin Grp, London, England
[2] Univ London Imperial Coll Sci Technol & Med, Fac Med, MRC Clin Sci Ctr, Nucl Org Grp, London, England
[3] Inst Super Ciencia Saude Sul, Caparica, Portugal
基金
英国医学研究理事会;
关键词
transcription; epigenetic memory; pre-B cell;
D O I
10.1038/sj.embor.7400366
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Variant histone H3.3 is incorporated into nucleosomes by a mechanism that does not require DNA replication and has also been implicated as a potential mediator of epigenetic memory of active transcriptional states. In this study, we have used chromatin immunoprecipitation analysis to show that H3.3 is found mainly at the promoters of transcriptionally active genes. We also show that H3.3 combines with H3 acetylation and K4 methylation to form a stable mark that persists during mitosis. Our results suggest that H3.3 is deposited principally through the action of chromatin-remodelling complexes associated with transcriptional initiation, with deposition mediated by RNA polymerase II elongation having only a minor role.
引用
收藏
页码:354 / 360
页数:7
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