Nonexponential kinetics of a single tRNA(Phe) molecule under physiological conditions

被引：99

作者：

Jia, YW

Sytnik, A

Li, LQ

Vladimirov, S

Cooperman, BS

Hochstrasser, RM

机构：

[1] Chemistry Department, University of Pennsylvania, Philadelphia

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1997年 / 94卷 / 15期

关键词：

D O I：

10.1073/pnas.94.15.7932

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The fluorescence decay functions of individual, specifically labeled tRNA(Phe) molecules exhibit nonexponential character as a result of conformational dynamics occurring during the measurement on a single molecule. tRNA(Phe) conformational states that interchange much more slowly are evidenced by the distribution of lifetimes observed for many individual molecules. A structural model fur the nonexponential decay indicates that the tRNA(Phe)-probe adduct fluctuates between two states, one of which provides conditions that quench the probe fluorescence.

引用

收藏

页码：7932 / 7936

页数：5

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