In vitro assays for activities of retroviral integrase

被引：79

作者：

Chow, SA ^{[1
]}

机构：

[1] UNIV CALIF LOS ANGELES,SCH MED,INST MOL BIOL,LOS ANGELES,CA 90095

来源：

METHODS-A COMPANION TO METHODS IN ENZYMOLOGY | 1997年 / 12卷 / 04期

关键词：

D O I：

10.1006/meth.1997.0484

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Integration of retroviral DNA, an essential step during the retroviral life cycle, is mediated by the viral protein integrase. Simple in vitro assays for measuring integrase activities are described, including catalysis (3'-end processing, 3'-end joining, disintegration), juxtaposition of viral DNA ends, DNA binding, and target site selection. The described assays will be useful in elucidating the molecular mechanism of retroviral integration and screening for integrase inhibitors as potential anti-retroviral drugs. (C) 1997 Academic Press.

引用

页码：306 / 317

页数：12

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