Inactivation of the flax rust resistance gene M associated with loss of a repeated unit within the leucine-rich repeat coding region

被引:251
作者
Anderson, PA
Lawrence, GJ
Morrish, BC
Ayliffe, MA
Finnegan, EJ
Ellis, JG
机构
[1] CSIRO,DIV PLANT IND,CANBERRA,ACT 2601,AUSTRALIA
[2] COOPERAT RES CTR PLANT SCI,CANBERRA,ACT 2601,AUSTRALIA
关键词
D O I
10.1105/tpc.9.4.641
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The M rust resistance gene from flax was cloned after two separate approaches, an analysis of spontaneous M mutants with an L6 gene-derived DNA probe and tagging with the maize transposon Activator, independently identified the same gene. The gene encodes a protein of the nucleotide binding site leucine-rich repeat class and is related (86% nucleotide identity) to the unlinked L6 rust resistance gene. In contrast to the L locus, which contains a single gene with multiple alleles, similar to 15 related genes occur at the complex M locus, with only one encoding the M resistance specificity. The M protein contains two direct repeats of 147 and 149 amino acids in the C-terminal part of the leucine-rich region. Three mutant alleles of M encoding a product containing a single repeat unit of 154 amino acids were isolated. The mutant DNA sequences probably occurred by unequal intragenic exchange in the coding region of the repeats. The recombinant alleles lost M resistance and gained no detectable new resistance specificity.
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收藏
页码:641 / 651
页数:11
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