Sm-p80-based DNA vaccine formulation induces potent protective immunity against Schistosoma mansoni

被引：39

作者：

Ahmad, G. ^{[1
]}

Torben, W. ^{[1
]}

Zhang, W. ^{[1
]}

Wyatt, M. ^{[1
]}

Siddiqui, A. A. ^{[1
,2
]}

机构：

[1] Texas Tech Univ, Hlth Sci Ctr, Dept Microbiol & Immunol, Lubbock, TX 79430 USA

[2] Texas Tech Univ, Hlth Sci Ctr, Dept Internal Med, Lubbock, TX 79430 USA

来源：

PARASITE IMMUNOLOGY | 2009年 / 31卷 / 03期

关键词：

calpain; DNA vaccination; schistosomiasis; Schistosoma; Sm-p80; LARGE SUBUNIT; CALPAIN SM-P80; ANTIGEN; IMMUNIZATION; CANDIDATE; INFECTION; DISEASES; SURFACE; MODEL; STAGE;

D O I：

10.1111/j.1365-3024.2008.01091.x

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

No effective vaccine exists for the human parasitic disease, schistosomiasis. We have targeted a functionally important antigen, Sm-p80 as a vaccine candidate because of its consistent immunogenicity, protective potential and important role in the immune evasion process. In this study we report that a Sm-p80-based DNA vaccine formulation confers 59% reduction in worm burden in mice. Animals immunized with Sm-p80-pcDNA3 exhibited a decrease in egg production by 84%. Sm-p80 DNA elicited strong immune responses that include IgG2A and IgG2B antibody isotypes in vaccinated animals. Splenocytes proliferated in response to Sm-p80 produced appreciably more Th1 response enhancing cytokines (IL-2, IFN-gamma) than Th2 response enhancing cytokines (IL-4, IL-10). These data reinforce the potential of Sm-p80 as an excellent vaccine candidate for schistosomiasis.

引用

页码：156 / 161

页数：6

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