Comparison of the infectivity of isolates of Listeria monocytogenes following intragastric and intravenous inoculation in mice

被引:24
作者
Barbour, AH
Rampling, A
Hormaeche, CE
机构
[1] W DORSET HOSP, DEPT PATHOL, PUBL HLTH LAB, DORCHESTER DT1 2JY, DORSET, ENGLAND
[2] UNIV ADELAIDE, DEPT PATHOL, ADELAIDE, SA 5005, AUSTRALIA
[3] UNIV NEWCASTLE, SCH MED, DEPT MICROBIOL, NEWCASTLE UPON TYNE NE2 4HH, TYNE & WEAR, ENGLAND
关键词
Listeria monocytogenes; infectivity; mice; intravenous; intragastric;
D O I
10.1006/mpat.1996.0023
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
The infectivity of 19 haemolytic isolates of Listeria monocytogenes from different sources (clinical and environmental) and representative isolates of Listeria ivanovii and Listeria innocua was compared following intragastric (i.g.) and intravenous (i.v.) inoculation in immunocompetent male BALB/c mice. There was marked variation in the infectivity of the different isolates by either route but when isolates were ranked in descending order by spleen count, following i.g. administration, the strains fell into four groups. Infectivity of some isolates also differed when i.v. inoculation was compared with i.g. administration, so that assessment of virulence by spleen counts only following i.v. inoculation might fail to detect isolates of poor infectivity by the i.g. route. These results suggest that intragastric inoculation of normal immunocompetent mice is a useful model for detecting strains of L. monocytogenes that are poorly invasive via the gut even though they are relatively virulent by intravenous inoculation. (C) 1996 Academic Press Limited
引用
收藏
页码:247 / 253
页数:7
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