Placenta growth factor and vascular endothelial growth factor are co-expressed during early embryonic development

We have used the polymerase chain reaction to identify mouse proteins similar in primary structure to the endothelial cell mitogen Vascular Endothelial Growth Factor (VEGF). One amplified product encoded mouse Placenta Growth Factor (PlGF), The pattern of PlCF gene expression in mouse embryos was studied by in situ hybridization. Transcripts encoding mouse PlGF were abundant in trophoblastic giant cells associated with the parietal yolk sac at early stages of embryogenesis. VEGF transcripts were also detected in trophoblastic giant cells raising the possibility that these cells may secrete heterodimers consisting of one PlGF subunit and one VEGF subunit. The secretion of PlGF and VEGF by trophoblastic giant cells is likely to be the signal which initiates and co-ordinates vascularization in the deciduum and placenta during early embryogenesis.