Functional characterization of the S-cerevisiae genome by gene deletion and parallel analysis

被引：3155

作者：

Winzeler, EA

Shoemaker, DD

Astromoff, A

Liang, H

Anderson, K

Andre, B

Bangham, R

Benito, R

Boeke, JD

Bussey, H

Chu, AM

Connelly, C

Davis, K

Dietrich, F

Dow, SW

EL Bakkoury, M

Foury, F

Friend, SH

Gentalen, E

Giaever, G

Hegemann, JH

Jones, T

Laub, M

Liao, H

Liebundguth, N

Lockhart, DJ

Lucau-Danila, A

Lussier, M

M'Rabet, N

Menard, P

Mittmann, M

Pai, C

Rebischung, C

Revuelta, JL

Riles, L

Roberts, CJ

Ross-MacDonald, P

Scherens, B

Snyder, M

Sookhai-Mahadeo, S

Storms, RK

Véronneau, S

Voet, M

Volckaert, G

Ward, TR

Wysocki, R

Yen, GS

Yu, KX

Zimmermann, K

Philippsen, P

机构：

[1] Stanford Univ, Sch Med, Dept Biochem, Stanford, CA 94305 USA

[2] Rosetta Inpharmat Inc, Kirkland, WA 98034 USA

[3] Free Univ Brussels, Lab Physiol Cellulaire & Genet Levures, Brussels, Belgium

[4] Yale Univ, Dept Mol Cellular & Dev Biol, New Haven, CT 06520 USA

[5] Univ Salamanca, Dipartimento Microbiol & Genet, Edificio Dept 323, CSIC, E-37007 Salamanca, Spain

[6] Johns Hopkins Univ, Sch Med, Dept Mol Biol & Genet, Baltimore, MD 21205 USA

[7] McGill Univ, Dept Biol, Montreal, PQ H3A 1B1, Canada

[8] Univ Basel, Biozentrum, Dept Mol Microbiol, CH-4003 Basel, Switzerland

[9] ULB, IRMW, B-1070 Brussels, Belgium

[10] Catholic Univ Louvain, FYSA, B-1348 Louvain, Belgium

[11] Affymetrix, Santa Clara, CA 95051 USA

[12] Inst Microbiol, D-40225 Dusseldorf, Germany

[13] Washington Univ, Sch Med, Dept Genet, St Louis, MO 63110 USA

[14] Katholieke Univ Leuven, Lab Gene Technol, B-3001 Louvain, Belgium

来源：

SCIENCE | 1999年 / 285卷 / 5429期

关键词：

D O I：

10.1126/science.285.5429.901

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The functions of many open reading frames (ORFs) identified in genome-sequencing projects are unknown. New, whole-genome approaches are required to systematically determine their function. A total of 6925 Saccharomyces cerevisiae strains were constructed, by a high-throughput strategy, each with a precise deletion of one of 2026 ORFs (more than one-third of the ORFs in the genome). Of the deleted ORFs, 17 percent were essential for viability in rich medium. The phenotypes of more than 500 deletion strains were assayed in parallel. Of the deletion strains, 40 percent showed quantitative growth defects in either rich or minimal medium.

引用

页码：901 / 906

页数：6

共 39 条

[1]

Baganz F, 1998, YEAST, V14, P1417, DOI 10.1002/(SICI)1097-0061(199811)14:15<1417::AID-YEA334>3.3.CO

[2]

2-E

[3] Genome cross-referencing and XREFdb: Implications for the identification and analysis of genes mutated in human disease [J].