Yeast mitochondrial F1F0-ATPase: the novel subunit e is identical Tim11

We report here the identification of the novel subunit of the mitochondrial F1F0-ATPase from Saccharomyces cerevisiae, ATPase subunit e. Yeast ATPase subunit e displays significant similarities in both amino acid sequence, properties (hydropathy and predicted coiled-coil structure) and orientation in the inner membrane,,vith previously identified mammalian ATPase subunit e proteins, Estimation of its native molecular mass and ability to be co-immunoprecipitated with a subunit of the F-1-ATPase, demonstrate that subunit e is a subunit of the F1F0-ATPase. Stable expression of subunit e requires the presence of the mitochondrially encoded subunits of the F-0-ATPase, Subunit e had been previously identified as Tim11 and was proposed to be involved in the process of sorting of proteins to the mitochondrial inner membrane. (C) 1997 Federation of European Biochemical Societies.