On the reduction steps in the mevalonate independent 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway for isoprenoid biosynthesis in the bacterium Zymomonas mobilis

被引：27

作者：

Charon, L ^{[1
]}

Pale-Grosdemange, C ^{[1
]}

Rohmer, M ^{[1
]}

机构：

[1] Univ Strasbourg, CNRS, Inst Le Bel, F-67070 Strasbourg, France

来源：

TETRAHEDRON LETTERS | 1999年 / 40卷 / 40期

关键词：

D O I：

10.1016/S0040-4039(99)01531-2

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

Zymomonas mobilis synthesizes triterpenoids of the hopane series via the methylerythritol phosphate (MEP) pathway. Owing to the peculiar metabolic pathways in this bacterium, [1-H-2]glucose is the appropriate substrate for generating in vivo deuterium labeled NADP(2)H. After feeding of Z. mobilis with [1-H-2]glucose, deuterium labeling was found on all carbon atoms derived from C-2 and C-4 of isopentenyl diphosphate. The deuterium at C-4 arose from the NADPH dependent reduction catalyzed by the reducto-isomerase converting I-deoxy-D-xylulose 5-phosphate into 2-C-methyl-D-erythritol 4-phosphate. The deuterium at C-2 resulted from an additional reduction at a yet undetermined steps of the MEP biosynthetic pathway. (C) 1999 Published by Elsevier Science Ltd. All rights reserved.

引用

页码：7231 / 7234

页数：4

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