Chemically induced liver regeneration is characterized by specific IL-6 gene expression

被引：10

作者：

Salazar-Montes, A ^{[1
]}

Rincón, AR ^{[1
]}

Panduro, A ^{[1
]}

Armendáriz-Borunda, J ^{[1
]}

机构：

[1] Univ Guadalajara, CUCS, Inst Mol Biol Med, Guadalajara 44281, Jalisco, Mexico

来源：

HEPATOLOGY RESEARCH | 1999年 / 15卷 / 01期

关键词：

gene expression-regulation; RT-PCR; IL-6; liver regeneration; inflammation;

D O I：

10.1016/S1386-6346(98)00056-4

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

IL-6 and TNFx have been shown as important in! cytokines in liver regeneration induced in partially hepatectomized rats. Nonetheless, IL-6 gene expression in the course of chemically induced hepatic regeneration, resembling what occurs in humans infected by hepatitis virus in acute manner, remains obscure. To gain an insight on this matter He determined IL-6 and TNFx gene expression in Wistar rats after acute and chronic liver intoxication with CCl4, and/or turpentine. We used a semiquantitative method of RT-PCR to analyze cytokine gene expression and, quite interestingly, IL-6 was detected only at 23 h after acute damage as opposed to TNFx expression that uas increased from 6 to 48 h after acute injury. A single intradermally-injected dose of turpentine did not suffice to induce IL-C gene expression at any studied times, but, as expected. turpentine-treated animals showed decreasing ci albumin mRNA transcripts as compared with the control rats. Furthermnre. livers from cirrhotic animals did not show detectable IL-6 gene transcripts. but, IL-6 expression re-appealed in cirrhotic rats treated with an additional acute CCl4, doss, indicating that IL-6 is a critical component of the brisk regenerative response induced by CCl4, (C) 1999 Elsevier it Science Ireland Ltd. Ail rights reserved.

引用

页码：10 / 21

页数：12

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