The release of cytochrome c from mitochondria: A primary site for Bcl-2 regulation of apoptosis

被引：4204

作者：

Kluck, RM ^{[1
]}

BossyWetzel, E ^{[1
]}

Green, DR ^{[1
]}

Newmeyer, DD ^{[1
]}

机构：

[1] LA JOLLA INST ALLERGY & IMMUNOL,DIV CELLULAR IMMUNOL,SAN DIEGO,CA 92121

来源：

SCIENCE | 1997年 / 275卷 / 5303期

关键词：

D O I：

10.1126/science.275.5303.1132

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

In a cell-free apoptosis system, mitochondria spontaneously released cytochrome c, which activated DEVD-specific caspases, leading to fodrin cleavage and apoptotic nuclear morphology. Bcl-2 acted in situ on mitochondria to prevent the release of cytochrome c and thus caspase activation. During apoptosis in intact cells, cytochrome c translocation was similarly blocked by Bcl-2 but not by a caspase inhibitor, zVAb-fmk. In vitro, exogenous cytochrome c bypassed the inhibitory effect of Bcl-2. Cytochrome c release was unaccompanied by changes in mitochondrial membrane potential. Thus, Bcl-2 acts to inhibit cytochrome c translocation, thereby blocking caspase activation and the apoptotic process.

引用

页码：1132 / 1136

页数：5

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