Vp165 and GLUT4 share similar vesicle pools along their trafficking pathways in rat adipose cells

被引：47

作者：

Malide, D ^{[1
]}

StDenis, JF ^{[1
]}

Keller, SR ^{[1
]}

Cushman, SW ^{[1
]}

机构：

[1] DARTMOUTH COLL SCH MED,DEPT BIOCHEM,HANOVER,NH 03755

来源：

FEBS LETTERS | 1997年 / 409卷 / 03期

关键词：

GLUT4; vp165; adipose cell; wortmannin; confocal microscopy; insulin action;

D O I：

10.1016/S0014-5793(97)00563-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

vp165 (or gp160) is an aminopeptidase that has been identified as one of the major proteins of the GLUT4-containing vesicles, In the present study we have determined the degree of co-localization between vp165 and GLUT4 in rat adipose cells and used perturbation by wortmannin to assess the exocytic and endocytic steps along the translocation and recycling pathways of GLUT4 in the absence and presence of insulin, Western blots of subcellular membrane fractions demonstrate very similar distributions of vp165 and GLUT4, Confocal microscopy of whole cells provides direct evidence that these proteins share the same vesicle populations moving both towards and from the plasma membrane. These data are consistent with the presence of a distinct insulin-sensitive compartment that sequesters both GLUT4 and vp165 and suggest similar trafficking routes through the recycling compartments. (C) 1997 Federation of European Biochemical Societies.

引用

页码：461 / 468

页数：8

共 36 条

[1] ROLE FOR PHOSPHATIDYLINOSITOL 3-KINASE IN THE SORTING AND TRANSPORT OF NEWLY SYNTHESIZED LYSOSOMAL-ENZYMES IN MAMMALIAN-CELLS [J].