The structure of I-CreI, a Group I intron-encoded homing endonuclease

被引:107
作者
Heath, PJ
Stephens, KM
Monnat, RJ
Stoddard, BL
机构
[1] FRED HUTCHINSON CANC RES CTR,PROGRAM STRUCT BIOL,DIV BASIC SCI,SEATTLE,WA 98104
[2] UNIV WASHINGTON,DEPT PATHOL,SEATTLE,WA 98195
关键词
D O I
10.1038/nsb0697-468
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The structure of I-Crel provides the first view of a protein encoded by a gene within an intron. This endonuclease recognizes a long DNA site similar to 20 base pairs in length and facilitates the lateral transfer of that intron. The protein exhibits a DNA-binding surface consisting of four antiparallel beta-strands that form a 20 A wide groove which is over 70 Angstrom long, The architecture of this fold is different from that of the TATA binding protein, TBP, which also contains an antiparallel beta-saddle. The conserved LACLIDADG motif, which is found in many mobile intron endonucleases, maturases and inteins, forms a novel helical interface and contributes essential residues to the active site.
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页码:468 / 476
页数:9
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