Evidence for neuropeptide FF (FLFQPQRFamide) in rat dorsal root ganglia

被引：19

作者：

Allard, M

Rousselot, P

Lombard, MC

Theodosis, DT

机构：

[1] Univ Bordeaux 2, INSERM, U378, Inst Francois Magendie, F-33076 Bordeaux, France

[2] INSERM, U161, F-75014 Paris, France

[3] EPHE, Lab Physiopharmacol Douleur, F-75014 Paris, France

来源：

PEPTIDES | 1999年 / 20卷 / 03期

关键词：

neuropeptide FF; sensory afferents; nociception; radioimmunoassay; immunohistochemistry;

D O I：

10.1016/S0196-9781(99)00039-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

By using specific antibodies and radioimmunological and immunohistochemical methods, we here show that neuropeptide FF (NPFF) occurs in cervical and lumbar dorsal root ganglia cells. Levels in the ganglia were low because they were detectable only after colchicine treatment or after unilateral dorsal rhizotomy. Similar high-performance liquid chromatography profiles were obtained from dorsal root ganglia and spinal cord extracts, indicating that the NPFF-immunoreactivity in the dorsal root ganglia represented similar molecular forms to that in the spinal cord. Immunocytochemistry localized NPFF-immunoreactivity in small- and medium-sized cells. These data suggest that low levels of NPFF present in fine diameter primary afferent fibers could be involved in the treatment of nociceptive information from fore- or hindlimb. (C) 1999 Elsevier Science Inc. All rights reserved.

引用

页码：327 / 333

页数：7

共 31 条

[1] CHARACTERIZATION AND LOCALIZATION OF A PUTATIVE MORPHINE-MODULATING PEPTIDE, FLFQPQRFAMIDE, IN THE RAT SPINAL-CORD - BIOCHEMICAL AND IMMUNOCYTOCHEMICAL STUDIES [J].