Gene expression of proteins influencing the calcium homeostasis in patients with persistent and paroxysmal atrial fibrillation

被引:133
作者
Brundel, BJJM
Van Gelder, IC
Henning, RH
Tuinenburg, AE
Deelman, LE
Tieleman, RG
Crandjean, JG
Van GIlst, WH
Crijns, HJGM
机构
[1] Univ Groningen Hosp, Dept Cardiol, Thoraxctr, NL-9700 RB Groningen, Netherlands
[2] Univ Groningen Hosp, Dept Clin Pharmacol, Thoraxctr, NL-9700 RB Groningen, Netherlands
[3] Univ Groningen Hosp, Dept Thorac Surg, Thoraxctr, NL-9700 RB Groningen, Netherlands
关键词
atrial fibrillation; Ca2+-handling proteins; gene expression;
D O I
10.1016/S0008-6363(99)00045-0
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Objective: Persistent atrial fibrillation (AF) results in an impairment of atrial function. In order to elucidate the mechanism behind this phenomenon, we investigated the gene expression of proteins influencing calcium handling. Methods: Right atrial appendages were obtained from eight patients with paroxysmal AF, ten with persistent AF (>8 months) and 18 matched controls in sinus rhythm. All controls underwent coronary artery bypass grafting, whereas most AF patients underwent Cox's MAZE surgery (n=12). All patients had a normal left ventricular function. Total RNA was isolated and reversely transcribed into cDNA. In a semi-quantitative polymerase chain reaction the cDNA of interest and of glyceraldehyde-3-phosphate dehydrogenase were coamplified and separated by ethidium bromide-stained gel electrophoresis. Slot blot analysis was performed to study protein expression. Results: L-type calcium channel alpha(1) and sarcoplasmic reticulum Ca2+-ATPase mRNA (-57%, p=0.01 and -28%, p=0.04, respectively) and protein contents (-43%, p=0.02 and -28%, p=0.04, respectively) were reduced in patients with persistent AF compared to the controls, mRNA contents of phospholamban, ryanodine receptor type 2 and sodium/calcium exchanger were comparable. No changes were observed in patients with paroxysmal AF. Conclusions: Alterations in gene expression of proteins involved in the calcium homeostasis occur only in patients with long-term persistent AF. In the absence of underlying heart disease, the changes are rather secondary than primary to AF. (C) 1999 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:443 / 454
页数:12
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