Anisylazoformylarginine: A superior assay substrate for carboxypeptidase B type enzymes

被引：14

作者：

Mock, WL ^{[1
]}

Stanford, DJ ^{[1
]}

机构：

[1] Univ Illinois, Dept Chem, Chicago, IL 60607 USA

来源：

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 2002年 / 12卷 / 08期

关键词：

D O I：

10.1016/S0960-894X(02)00128-2

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

Anisylazoformylarginine (CH3OC6H4-N=N-CO-Arg-OH) is rapidly hydrolyzed at the acyl-arginine linkage by the zinc-enzyme porcine carboxypeptidase B. The catalytic reaction is readily monitored spectrophotometrically by disappearance of the intense absorption (349 nm, epsilon 19,100) of the azo chromophore, which chemically fragments after substrate cleavage. (C) 2002 Elsevier Science Ltd. All rights reserved.

引用

页码：1193 / 1194

页数：2

共 7 条

[1] Both cellular and soluble forms of thrombomodulin inhibit fibrinolysis by potentiating the activation of thrombin-activable fibrinolysis inhibitor [J].