The mammalian gene function resource: the international knockout mouse consortium

被引：232

作者：

Bradley, Allan ^{[1
]}

Anastassiadis, Konstantinos ^{[2
]}

Ayadi, Abdelkader ^{[3
,4
]}

Battey, James F. ^{[5
]}

Bell, Cindy ^{[6
]}

Birling, Marie-Christine ^{[3
,4
]}

Bottomley, Joanna ^{[1
]}

Brown, Steve D. ^{[7
]}

Buerger, Antje ^{[8
]}

Bult, Carol J. ^{[9
]}

Bushell, Wendy ^{[1
]}

Collins, Francis S. ^{[5
]}

Desaintes, Christian ^{[10
]}

Doe, Brendan ^{[11
]}

Economides, Aris ^{[12
]}

Eppig, Janan T. ^{[9
]}

Finnell, Richard H. ^{[13
,14
]}

Fletcher, Colin ^{[15
]}

Fray, Martin ^{[7
]}

Frendewey, David ^{[12
]}

Friedel, Roland H. ^{[8
,16
]}

Grosveld, Frank G. ^{[17
]}

Hansen, Jens ^{[8
]}

Herault, Yann ^{[3
,4
]}

Hicks, Geoffrey ^{[18
]}

Hoerlein, Andreas ^{[8
]}

Houghton, Richard ^{[1
]}

de Angelis, Martin Hrabe ^{[19
]}

Huylebroeck, Danny ^{[20
]}

Iyer, Vivek ^{[1
]}

de Jong, Pieter J. ^{[21
]}

Kadin, James A. ^{[9
]}

Kaloff, Cornelia ^{[8
]}

Kennedy, Karen ^{[1
]}

Koutsourakis, Manousos ^{[1
]}

Lloyd, K. C. Kent ^{[22
]}

Marschall, Susan ^{[19
]}

Mason, Jeremy ^{[9
]}

McKerlie, Colin ^{[23
]}

McLeod, Michael P. ^{[13
]}

von Melchner, Harald ^{[24
]}

Moore, Mark ^{[15
]}

Mujica, Alejandro O. ^{[1
,12
]}

Nagy, Andras ^{[25
]}

Nefedov, Mikhail ^{[21
]}

Nutter, Lauryl M. ^{[23
]}

Pavlovic, Guillaume ^{[3
,4
]}

Peterson, Jane L. ^{[15
]}

Pollock, Jonathan ^{[26
]}

Ramirez-Solis, Ramiro ^{[1
]}

机构：

[1] Wellcome Trust Sanger Inst, Cambridge CB10 1HH, England

[2] Tech Univ Dresden, Biotechnol Ctr BIOTEC, D-01307 Dresden, Germany

[3] Inst Clin Souris, F-67404 Illkirch Graffenstaden, France

[4] Inst Genet & Biol Mol & Cellulaire, F-67404 Illkirch Graffenstaden, France

[5] Natl Inst Deafness & Other Commun Disorders, NIH, Bethesda, MD 20892 USA

[6] Genome Canada, Ottawa, ON K2P 1P1, Canada

[7] MRC Harwell, Mammalian Genet Unit, Didcot OX11 0RD, Oxon, England

[8] Tech Univ Munich, Helmholtz Zentrum Munchen, Inst Dev Genet, D-85764 Neuherberg, Germany

[9] Jackson Lab, Bar Harbor, ME 04609 USA

[10] DG Res & Innovat, European Commiss, B-1049 Brussels, Belgium

[11] CNR, Ist Biol Cellulare, I-00015 Rome, Italy

[12] Regeneron Pharmaceut Inc, Velocigene Div, Tarrytown, NY 10591 USA

[13] Texas A&M Inst Genom Med, College Stn, TX 77843 USA

[14] Univ Texas Austin, Austin, TX 78712 USA

[15] NIH, Bethesda, MD 20205 USA

[16] Mt Sinai Hosp, Icahn Med Inst, New York, NY 10029 USA

[17] Erasmus MC, Dept Cell Biol, Ctr Biomed Genet, NL-3015 GE Rotterdam, Netherlands

[18] Univ Manitoba, Manitoba Inst Cell Biol, Winnipeg, MB R3E OV9, Canada

[19] Helmholtz Zentrum Munchen, Inst Expt Genet, D-85764 Neuherberg, Germany

[20] Univ Leuven KU Leuven, Dept Dev & Regenerat, Fac Med, B-3000 Louvain, Belgium

[21] CHORI, Oakland, CA 94609 USA

[22] Univ Calif Davis, Sch Vet Med, Mouse Biol Program, Davis, CA 95616 USA

[23] Hosp Sick Children, SickKids Fdn, Res Inst, Toronto, ON M5G 2L3, Canada

[24] Univ Frankfurt Med Sch, Dept Mol Haematol, D-60590 Frankfurt, Germany

[25] Mt Sinai Hosp, Samuel Lunenfeld Res Inst, Toronto, ON M5G 1X5, Canada

[26] NIDA, Div Basic Neurosci & Res, Bethesda, MD 20892 USA

[27] Univ Calgary, Dept Biochem & Mol Biol, Calgary, AB T2N 1N4, Canada

[28] EMBL, I-00015 Rome, Italy

[29] Charite, Dept Vertebrate Genom, Ctr Cardiovasc Res, D-10115 Berlin, Germany

[30] Univ Cambridge, Dept Physiol Dev & Neurosci, Cambridge CB2 3EG, England

[31] Univ British Columbia, Child & Family Res Inst, Dept Med Genet, Ctr Mol Med & Therapeut, Vancouver, BC V5Z 4H4, Canada

[32] EBI, Cambridge CB10 1ST, England

[33] Ottawa Hosp Res Inst, Ottawa, ON K1N 6N5, Canada

[34] Kumamoto Univ, Inst Resource Dev & Anal, Div Dev Genet, Ctr Anim Resources & Dev, Kumamoto 8600811, Japan

[35] Max Planck Inst Psychiat, D-80804 Munich, Germany

[36] Deutsch Zentrum Neurodegenerat Erkrankungen eV DZ, D-80336 Munich, Germany

来源：

MAMMALIAN GENOME | 2012年 / 23卷 / 9-10期

基金：

美国国家卫生研究院;

关键词：

EMBRYONIC STEM-CELLS; GENOME;

D O I：

10.1007/s00335-012-9422-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In 2007, the International Knockout Mouse Consortium (IKMC) made the ambitious promise to generate mutations in virtually every protein-coding gene of the mouse genome in a concerted worldwide action. Now, 5 years later, the IKMC members have developed high-throughput gene trapping and, in particular, gene-targeting pipelines and generated more than 17,400 mutant murine embryonic stem (ES) cell clones and more than 1,700 mutant mouse strains, most of them conditional. A common IKMC web portal (www.knockoutmouse.org) has been established, allowing easy access to this unparalleled biological resource. The IKMC materials considerably enhance functional gene annotation of the mammalian genome and will have a major impact on future biomedical research.

引用

页码：580 / 586

页数：7

共 10 条

[1] The knockout mouse project [J].

Austin, CP ;

Battey, JF ;

Bradley, A ;

Bucan, M ;

Capecchi, M ;

Collins, FS ;

Dove, WF ;

Duyk, G ;

Dymecki, S ;

Eppig, JT ;

Grieder, FB ;

Heintz, N ;

Hicks, G ;

Insel, TR ;

Joyner, A ;

Koller, BH ;

Lloyd, KCK ;

Magnuson, T ;

Moore, MW ;

Nagy, A ;

Pollock, JD ;

Roses, AD ;

Sands, AT ;

Seed, B ;

Skarnes, WC ;

Snoddy, J ;

Soriano, P ;

Stewart, DJ ;

Stewart, F ;

Stillman, B ;

Varmus, H ;

Varticovski, L ;

Verma, IM ;

Vogt, TF ;

von Melchner, H ;

Witkowski, J ;

Woychik, RP ;

Wurst, W ;

Yancopoulos, GD ;

Young, SG ;

Zambrowicz, B .

NATURE GENETICS, 2004, 36 (09) :921-924

[2] The European dimension for the mouse genome mutagenesis program [J].

Auwerx, J ;

Avner, P ;

Baldock, R ;

Ballabio, A ;

Balling, R ;

Barbacid, M ;

Berns, A ;

Bradley, A ;

Brown, S ;

Carmeliet, P ;

Chambon, P ;

Cox, R ;

Davidson, D ;

Davies, K ;

Duboule, D ;

Forejt, J ;

Granucci, F ;

Hastie, N ;

de Angelis, MH ;

Jackson, I ;

Kioussis, D ;

Kollias, G ;

Lathrop, M ;

Lendahl, U ;

Malumbres, M ;

von Melchner, H ;

Müller, W ;

Partanen, J ;

Ricciardi-Castagnoli, P ;

Rigby, P ;

Rosen, B ;

Rosenthal, N ;

Skarnes, B ;

Stewart, AF ;

Thornton, J ;

Tocchini-Valentini, G ;

Wagner, E ;

Wahli, W ;

Wurst, W .

NATURE GENETICS, 2004, 36 (09) :925-927

[3] A mouse for all reasons [J].

Collins, Francis S. .

CELL, 2007, 128 (01) :9-13

[4] Dual RMCE for efficient re-engineering of mouse mutant alleles [J].

Osterwalder, Marco ;

Galli, Antonella ;

Rosen, Barry ;

Skarnes, William C. ;

Zeller, Rolf ;

Lopez-Rios, Javier .

NATURE METHODS, 2010, 7 (11) :893-+

[5] Agouti C57BL/6N embryonic stem cells for mouse genetic resources [J].

Pettitt, Stephen J. ;

Liang, Qi ;

Rairdan, Xin Y. ;

Moran, Jennifer L. ;

Prosser, Haydn M. ;

Beier, David R. ;

Lloyd, Kent C. ;

Bradley, Allan ;

Skarnes, William C. .

NATURE METHODS, 2009, 6 (07) :493-U34

[6] The IKMC web portal: a central point of entry to data and resources from the International Knockout Mouse Consortium [J].

Ringwald, Martin ;

Iyer, Vivek ;

Mason, Jeremy C. ;

Stone, Kevin R. ;

Tadepally, Hamsa D. ;

Kadin, James A. ;

Bult, Carol J. ;

Eppig, Janan T. ;

Oakley, Darren J. ;

Briois, Sebastien ;

Stupka, Elia ;

Maselli, Vincenza ;

Smedley, Damian ;

Liu, Songyan ;

Hansen, Jens ;

Baldock, Richard ;

Hicks, Geoff G. ;

Skarnes, William C. .

NUCLEIC ACIDS RESEARCH, 2011, 39 :D849-D855

[7] Resources for proteomics in mouse embryonic stem cells [J].

Schnuetgen, Frank ;

Ehrmann, Franziska ;

Poser, Ina ;

Hubner, Nina C. ;

Hansen, Jens ;

Floss, Thomas ;

deVries, Ingrid ;

Wurst, Wolfgang ;

Hyman, Anthony ;

Mann, Matthias ;

von Melchner, Harald .

NATURE METHODS, 2011, 8 (02) :103-104

[8] A conditional knockout resource for the genome-wide study of mouse gene function [J].

Skarnes, William C. ;

Rosen, Barry ;

West, Anthony P. ;

Koutsourakis, Manousos ;

Bushell, Wendy ;

Iyer, Vivek ;

Mujica, Alejandro O. ;

Thomas, Mark ;

Harrow, Jennifer ;

Cox, Tony ;

Jackson, David ;

Severin, Jessica ;

Biggs, Patrick ;

Fu, Jun ;

Nefedov, Michael ;

de Jong, Pieter J. ;

Stewart, A. Francis ;

Bradley, Allan .

NATURE, 2011, 474 (7351) :337-U361

[9] High-throughput engineering of the mouse genome coupled with high-resolution expression analysis [J].

Valenzuela, DM ;

Murphy, AJ ;

Frendewey, D ;

Gale, NW ;

Economides, AN ;

Auerbach, W ;

Poueymirou, WT ;

Adams, NC ;

Rojas, J ;

Yasenchak, J ;

Chernomorsky, R ;

Boucher, M ;

Elsasser, AL ;

Esau, L ;

Zheng, J ;

Griffiths, JA ;

Wang, XR ;

Su, H ;

Xue, YZ ;

Dominguez, MG ;

Noguera, I ;

Torres, R ;

Macdonald, LE ;

Stewart, AF ;

DeChiara, TM ;

Yancopoulos, GD .

NATURE BIOTECHNOLOGY, 2003, 21 (06) :652-659

[10] Manipulating the mouse genome to engineer precise functional syntenic replacements with human sequence [J].

Wallace, Helen A. C. ;

Marques-Kranc, Fatima ;

Richardson, Melville ;

Luna-Crespo, Francisco ;

Sharpe, Jackie A. ;

Hughes, Jim ;

Wood, William G. ;

Higgs, Douglas R. ;

Smith, Andrew J. H. .

CELL, 2007, 128 (01) :197-209

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