Novel method for rapid determination of clarithromycin sensitivity in Helicobacter pylori

被引：58

作者：

Gibson, JR

Saunders, NA

Burke, B

Owen, RJ

机构：

[1] Cent Publ Hlth Lab, Helicobacter Reference Unit, Lab Enter Pathogens, London NW9 5HT, England

[2] Cent Publ Hlth Lab, Div Virus Reference, Mol Biol Unit, London NW9 5HT, England

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 1999年 / 37卷 / 11期

关键词：

D O I：

10.1128/JCM.37.11.3746-3748.1999

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A novel PCR-hybridization assay, performed in single closed capillaries, was developed to detect clarithromycin resistance-associated gene mutations in Helicobacter pylori. Mutations were detected by thermal analysis in 33 of 34 (97%) resistant isolates but not in 66 isolates determined to be sensitive by conventional antibiotic assays. The method aas rapid and reproducible, and it reduced PCR product contamination risk.

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页码：3746 / 3748

页数：3

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