Effects of Amyloid β-Peptides and Gangliosides on Mouse Neural Stem Cells

被引：14

作者：

Itokazu, Yutaka ^{[1
,2
,3
]}

Kato-Negishi, Midori ^{[1
,2
,3
]}

Nakatani, Yoshihiko ^{[1
,2
,3
]}

Ariga, Toshio ^{[1
,2
,3
]}

Yu, Robert K. ^{[1
,2
,3
]}

机构：

[1] Georgia Regents Univ, Med Coll Georgia, Inst Mol Med & Genet, Augusta, GA 30912 USA

[2] Georgia Regents Univ, Med Coll Georgia, Inst Neurosci, Augusta, GA 30912 USA

[3] Charlie Norwood VA Med Ctr, Augusta, GA 30904 USA

来源：

NEUROCHEMICAL RESEARCH | 2013年 / 38卷 / 10期

关键词：

Alzheimer's disease; Amyloid beta-peptide; Ganglioside; Neural stem cell; Cell proliferation; INCREASED HIPPOCAMPAL NEUROGENESIS; ALZHEIMERS-DISEASE; GD3; GANGLIOSIDE; DEVELOPMENTAL-CHANGES; BRAIN GANGLIOSIDES; PROGENITOR CELLS; PROLIFERATION; DISRUPTION; PHENOTYPE; A-BETA-42;

D O I：

10.1007/s11064-013-1108-y

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The interaction of amyloid beta-proteins (A beta s) with membrane lipids has been postulated as an early event in A beta fibril formation in Alzheimer's disease. We evaluated the effects of several putative bioactive A beta s and gangliosides on neural stem cells (NSCs) isolated from embryonic mouse brains or the subventricular zone of adult mouse brains. Incubation of the isolated NSCs with soluble A beta 1-40 alone did not cause any change in the number of NSCs, but soluble A beta 1-42 increased their number. Aggregated A beta 1-40 and A beta 1-42 increased the number of NSCs but soluble and aggregated A beta 25-35 decreased the number. Soluble A beta 1-40 and A beta 1-42 did not affect the number of apoptotic cells but aggregated A beta 1-40 and A beta 1-42 did. When NSCs were treated with a combination of GM1 or GD3 and soluble A beta 1-42, cell proliferation was enhanced, indicating that both GM1 and GD3 as well as A beta s are involved in promoting cell proliferation and survival of NSCs. These observations suggest the potential of beneficial effects of using gangliosides and A beta s for promoting NSC proliferation.

引用

页码：2019 / 2027

页数：9

共 57 条

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