Highly Efficient Genome Modifications Mediated by CRISPR/Cas9 in Drosophila

被引：305

作者：

Yu, Zhongsheng ^{[1
,4
]}

Ren, Mengda ^{[2
]}

Wang, Zhanxiang ^{[3
]}

Zhang, Bo ^{[3
]}

Rong, Yikang S. ^{[5
]}

Jiao, Renjie ^{[1
]}

Gao, Guanjun ^{[2
]}

机构：

[1] Chinese Acad Sci, Inst Biophys, State Key Lab Brain & Cognit Sci, Beijing 100101, Peoples R China

[2] Tsinghua Univ, Sch Life Sci, Beijing 100084, Peoples R China

[3] Peking Univ, Coll Life Sci, Minist Educ, Key Lab Cell Proliferat & Differentiat, Beijing 100871, Peoples R China

[4] Univ Chinese Acad Sci, Beijing 100080, Peoples R China

[5] NCI, Lab Biochem & Mol Biol, NIH, Bethesda, MD 20892 USA

来源：

GENETICS | 2013年 / 195卷 / 01期

基金：

中国国家自然科学基金;

关键词：

GUIDED CAS9 NUCLEASE; GENE; MELANOGASTER; TALEN;

D O I：

10.1534/genetics.113.153825

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

We report that Cas9/gRNA mediates efficient genetic modifications in Drosophila. Through targeting seven loci, we achieved a germline efficiency of up to 100%. Genes in both heterochromatin and euchromatin can be modified efficiently. Thus the Cas9/gRNA system is an attractive tool for rapid disruption of essentially any gene in Drosophila.

引用

页码：289 / +

页数：17

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