Maturing reticulocytes internalize plasma membrane in glycophorin A-containing vesicles that fuse with autophagosomes before exocytosis

被引：148

作者：

Griffiths, Rebecca E. ^{[1
]}

Kupzig, Sabine ^{[1
]}

Cogan, Nicola ^{[1
]}

Mankelow, Tosti J. ^{[1
]}

Betin, Virginie M. S. ^{[2
]}

Trakarnsanga, Kongtana

Massey, Edwin J.

Lane, Jon D. ^{[2
]}

Parsons, Stephen F. ^{[1
]}

Anstee, David J. ^{[1
]}

机构：

[1] NHSBT, BITS, Bristol BS34 7QH, Avon, England

[2] Univ Bristol, Sch Med & Vet Sci, Dept Biochem, Cell Biol Labs, Bristol BS8 1TD, Avon, England

来源：

BLOOD | 2012年 / 119卷 / 26期

基金：

英国生物技术与生命科学研究理事会; 英国惠康基金;

关键词：

RED-BLOOD-CELLS; HUMAN ERYTHROCYTES; MONOCLONAL-ANTIBODIES; MATURATION; VACUOLES; CALRETICULIN; TRANSFUSION; ENDOCYTOSIS; CHAPERONES; GENERATION;

D O I：

10.1182/blood-2011-09-376475

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

The erythrocyte is one of the best characterized human cells. However, studies of the process whereby human reticulocytes mature to erythrocytes have been hampered by the difficulty of obtaining sufficient numbers of cells for analysis. In the present study, we describe an in vitro culture system producing milliliter quantities of functional mature human adult reticulocytes from peripheral blood CD34(+) cells. We show that the final stage of reticulocyte maturation occurs by a previously undescribed mechanism in which large glycophorin A-containing vesicles forming at the cytosolic face of the plasma membrane are internalized and fuse with autophagosomes before expulsion of the autophagosomal contents by exocytosis. Early reticulocyte maturation is characterized by the selective elimination of unwanted plasma membrane proteins (CD71, CD98, and beta 1 integrin) through the endosome-exosome pathway. In contrast, late maturation is characterized by the generation of large glycophorin A-decorated vesicles of autophagic origin. (Blood. 2012;119(26):6296-6306)

引用

页码：6296 / 6306

页数：11

共 35 条

[1] Generation of monoclonal antibodies to integrin-associated proteins - Evidence that alpha(3)beta(1) complexes with EMMPRIN/basigin/OX47/M6 [J].