Fast forward genetics based on virus-induced gene silencing

被引：476

作者：

Baulcombe, DC ^{[1
]}

机构：

[1] John Innes Ctr Plant Sci Res, Sainsbury Lab, Norwich NR4 7UH, Norfolk, England

来源：

CURRENT OPINION IN PLANT BIOLOGY | 1999年 / 2卷 / 02期

关键词：

D O I：

10.1016/S1369-5266(99)80022-3

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Gene expression in plants can be suppressed in a sequence-specific manner by infection with virus vectors carrying fragments of host genes, Recent developments have revealed that the mechanism of this gene silencing is based on an RNA-mediated defence against viruses. It has also emerged that a related mechanism is involved in the post-transcriptional silencing that accounts for between line variation in transgene expression and cosuppresion of transgenes and endogenous genes, The technology of virus-induced gene silencing is being refined and adapted as a high throughput procedure for functional genomics in plants.

引用

页码：109 / 113

页数：5

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