The Nicotiana tabacum plasma membrane aquaporin NtAQP1 is mercury-insensitive and permeable for glycerol

被引：184

作者：

Biela, A ^{[1
]}

Grote, K ^{[1
]}

Otto, B ^{[1
]}

Hoth, S ^{[1
]}

Hedrich, R ^{[1
]}

Kaldenhoff, R ^{[1
]}

机构：

[1] Univ Wurzburg, Julius von Sachs Inst Biowissensch, D-97082 Wurzburg, Germany

来源：

PLANT JOURNAL | 1999年 / 18卷 / 05期

关键词：

D O I：

10.1046/j.1365-313X.1999.00474.x

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

A new aquaporin from Nicotiana tabacum (cv. Samsun) was characterized. It shares sequence homology to the Arabidopsis thaliana PIP1 protein family. By two-phase partitioning and immunoblot analysis, plasma membrane localization could be demonstrated. The corresponding mRNA is highly abundant in roots and flowers, while it is rarely expressed in leaves and stems. Functional expression in Xenopus oocytes revealed that NtAQP1 can mediate glycerol transport in addition to water flow. However, NtAQP1 is impermeable for Na+, K+ and Cl- ions. The water permeability and selectivity could not be modulated by addition of mercurials or the activity of cAMP-dependent protein kinases.

引用

页码：565 / 570

页数：6

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