Peptide ligation and its application to protein engineering

被引：81

作者：

Cotton, GJ ^{[1
]}

Muir, TW ^{[1
]}

机构：

[1] Rockefeller Univ, Lab Synthet Prot Chem, New York, NY 10021 USA

来源：

CHEMISTRY & BIOLOGY | 1999年 / 6卷 / 09期

关键词：

D O I：

10.1016/S1074-5521(99)80109-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The ability to assemble a target protein from a series of peptide fragments, either synthetic or biosynthetic in origin, enables the covalent structure of a protein to be modified in an unprecedented fashion. The present technologies available for performing such peptide ligations are discussed, with an emphasis on how these methodologies have been utilized in protein engineering to investigate biological processes.

引用

页码：R247 / R256

页数：10

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