Inhibition of ozone-induced lung neutrophilia and nuclear factor-κB binding activity by vitamin A in rat

被引：16

作者：

Hisada, T ^{[1
]}

Adcock, IM ^{[1
]}

Nasuhara, Y ^{[1
]}

Salmon, M ^{[1
]}

Huang, TJ ^{[1
]}

Barnes, PJ ^{[1
]}

Chung, KF ^{[1
]}

机构：

[1] Natl Heart & Lung Inst, Imperial Coll Sch Med, Dept Thorac Med, London SW3 6LY, England

来源：

EUROPEAN JOURNAL OF PHARMACOLOGY | 1999年 / 377卷 / 01期

关键词：

NF-kappa B (nuclear factor-kappa B); transcription factor; asthma; neutrophilic inflammation; ozone;

D O I：

10.1016/S0014-2999(99)00405-7

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Vitamin A binds to retinoic acid receptors, which in turn may interact with other transcription factors. We determined its effect (2500 and 5000 IU/kg) on nuclear factor-kappa B binding activity in the lung, airway inflammation and bronchial hyperresponsiveness in rats exposed to ozone. Ozone (3 ppm, 3 h) caused neutrophil influx into bronchoalveolar lavage fluid (16.2 +/- 0.8 x 10(5) cells/ml, p < 0.01) and bronchial hyperresponsiveness (-logPC(200)ACh = 2.54 +/- 0.19, p < 0.05, compared to control animals, respectively). Vitamin A inhibited this neutrophilia dose-dependently together with the increased DNA-binding activity of nuclear factor-kappa B in lung extracts. Vitamin A did not affect bronchial hyperresponsiveness at both doses. Vitamin A inhibits ozone-induced neutrophilic inflammation through a reduction in nuclear factor-kappa B DNA binding activity. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：63 / 68

页数：6

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