EDARAVONE INHIBITS THE INDUCTION OF INOS GENE EXPRESSION AT TRANSCRIPTIONAL AND POSTTRANSCRIPTIONAL STEPS IN MURINE MACROPHAGES

被引:25
作者
Yoshida, Hideyuki [2 ]
Kwon, A-Hon [2 ]
Habara, Kozo [2 ]
Yamada, Masanori [2 ]
Kaibori, Masaki [2 ]
Kamiyama, Yasuo [2 ]
Nishizawa, Mikio [1 ,3 ]
Ito, Seiji [1 ]
Okumura, Tadayoshi [1 ]
机构
[1] Kansai Med Univ, Dept Med Chem, Moriguchi, Osaka 5708506, Japan
[2] Kansai Med Univ, Dept Surg, Moriguchi, Osaka 5708506, Japan
[3] Ritsumeikan Univ, Coll Life Sci, Dept Biomed Sci, Shiga, Japan
来源
SHOCK | 2008年 / 30卷 / 06期
关键词
Edaravone; iNOS; nuclear factor-kappa B; mRNA stabilization; antisense-transcript;
D O I
10.1097/SHK.0b013e318173ea0b
中图分类号
R4 [临床医学];
学科分类号
1002 ; 100602 ;
摘要
Edaravone, a free radical scavenger, plays crucial roles in the prevention of injuries to the brain, heart, and liver. Our in vivo study indicated that edaravone prevented endotoxin-induced liver injury through inhibition of NO production in addition to reductions in oxidative products and proinflammatory cytokine induction. Studies were performed to determine whether edaravone directly influences the induction of iNOS in murine RAW264 macrophages as a substitute for Kupffer cells (resident macrophages) in the liver. RAW264 cells were treated with LIPS (1 mu g/mL) in the presence or absence of edaravone. NO production, iNOS induction, and its related signaling were analyzed. Edaravone (0.5 - 5 mM) decreased the production of NO stimulated by LIPS in time- and dose-dependent manners, and these concentrations of edaravone had no cytotoxic effects. Edaravone decreased the levels of iNOS protein and mRNA. Transfection experiments with iNOS promoter luciferase constructs revealed that edaravone inhibited the activities of both iNOS promoter transactivation and iNOS mRNA stabilization. However, edaravone did not have any effects on I kappa B alpha degradation or nuclear factor-kappa B activation. In contrast, edaravone markedly suppressed the LPS-stimulated expression of iNOS antisense-transcript, which stabilizes iNOS mRNA by interacting with its S-untranslated region and RNA-binding proteins. Edaravone may inhibit the induction of iNOS gene expression at the steps of its promoter transactivation in a nuclear factor-kappa B-independent manner and mRNA stabilization in RAW264 cells.
引用
收藏
页码:734 / 739
页数:6
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