Involvement of PA/plasmin system in the processing of pro-MMP-9 and in the second step of pro-MMP-2 activation

被引：239

作者：

Baramova, EN

Bajou, K

Remacle, A

LHoir, C

Krell, HW

Weidle, UH

Noel, A

Foidart, JM

机构：

[1] UNIV LIEGE,BIOL LAB,B-4000 LIEGE,BELGIUM

[2] BOEHRINGER MANNHEIM GMBH,PENZBERG,GERMANY

来源：

FEBS LETTERS | 1997年 / 405卷 / 02期

关键词：

MMP-2; MMP-9; uPA/plasmin system; activation;

D O I：

10.1016/S0014-5793(97)00175-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Pro-MMP2 activation is a two-step process resulting in (1) an intermediate 64 kDa form generated by the MT1-MMP activity, and (2) a mature 62 kDa form. Addition of plasminogen to HT1080 cells cultured under various conditions, or to their membrane preparation, induced a complete conversion of the intermediate MMP-2 form to the mature one, and processing of pro-MMP-9. The pro-MMP-2 activation was inhibited by plasmin inhibitors and anti-uPA antibody. These results provide evidence for involvement of the PA/plasmin system in the second step of MMP-2 activation. (C) 1997 Federation of European Biochemical Societies.

引用

页码：157 / 162

页数：6

共 36 条

[1] Intermolecular autolytic cleavage can contribute to the activation of progelatinase A by cell membranes [J].