Self-organization of microtubules into bipolar spindles around artificial chromosomes in Xenopus egg extracts

被引：802

作者：

Heald, R ^{[1
]}

Tournebize, R ^{[1
]}

Blank, T ^{[1
]}

Sandaltzopoulos, R ^{[1
]}

Becker, P ^{[1
]}

Hyman, A ^{[1
]}

Karsenti, E ^{[1
]}

机构：

[1] EUROPEAN MOLEC BIOL LAB, GENE EXPRESS PROGRAM, D-69117 HEIDELBERG, GERMANY

来源：

NATURE | 1996年 / 382卷 / 6590期

关键词：

D O I：

10.1038/382420a0

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Functional nuclei and mitotic spindles are shown to assemble around DNA-coated beads incubated in Xenopus egg extracts. Bipolar spindles assemble in the absence of centrosomes and kinetochores, indicating that bipolarity is an intrinsic property of microtubules assembling around chromatin in a mitotic cytoplasm. Microtubules nucleated at dispersed sites with random polarity rearrange into two arrays of uniform polarity. Spindle-pole formation requires cytoplasmic dynein-dependent translocation of microtubules across one another. It is proposed that spindles form in the absence of centrosomes by motor-dependent sorting of microtubules according to their polarity.

引用

页码：420 / 425

页数：6

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