Rescue of influenza A virus from recombinant DNA

被引：645

作者：

Fodor, E

Devenish, L

Engelhardt, OG

Palese, P

Brownlee, GG

García-Sastre, A

机构：

[1] Univ Oxford, Sir William Dunn Sch Pathol, Chem Pathol Unit, Oxford OX1 3RE, England

[2] CUNY Mt Sinai Sch Med, Dept Microbiol, New York, NY 10029 USA

来源：

JOURNAL OF VIROLOGY | 1999年 / 73卷 / 11期

关键词：

D O I：

10.1128/JVI.73.11.9679-9682.1999

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We have rescued influenza A virus by transfection of 12 plasmids into Vero cells. The eight individual negative-sense genomic viral RNAs were transcribed from plasmids containing human RNA polymerase I promoter and hepatitis delta virus ribozyme sequences. The three influenza virus polymerase proteins and the nucleoprotein were expressed from protein expression plasmids. This plasmid-based reverse genetics technique facilitates the generation of recombinant influenza viruses containing specific mutations in their genes.

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页码：9679 / 9682

页数：4

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