A reversible gene trap collection empowers haploid genetics in human cells

被引：77

作者：

Buerckstuemmer, Tilmann ^{[1
]}

Banning, Carina ^{[1
]}

Hainzl, Philipp ^{[1
,2
]}

Schobesberger, Richard ^{[1
]}

Kerzendorfer, Claudia ^{[2
]}

Pauler, Florian M. ^{[2
]}

Chen, Doris ^{[2
]}

Them, Nicole ^{[2
]}

Schischlik, Fiorella ^{[2
]}

Rebsamen, Manuele ^{[2
]}

Smida, Michal ^{[2
]}

de la Cruz, Ferran Fece ^{[2
]}

Lapao, Ana ^{[1
,2
]}

Liszt, Melissa ^{[1
,2
]}

Eizinger, Benjamin ^{[1
]}

Guenzl, Philipp M. ^{[2
]}

Blomen, Vincent A. ^{[3
]}

Konopka, Tomasz ^{[2
]}

Gapp, Bianca ^{[2
]}

Parapatics, Katja ^{[2
]}

Maier, Barbara ^{[2
,4
]}

Stoeckl, Johannes ^{[5
]}

Fischl, Wolfgang ^{[1
]}

Salic, Sejla ^{[1
]}

Casari, M. Rita Taba ^{[1
]}

Knapp, Sylvia ^{[2
,4
]}

Bennett, Keiryn L. ^{[2
]}

Bock, Christoph ^{[2
]}

Colinge, Jacques ^{[2
]}

Kralovics, Robert ^{[2
]}

Ammerer, Gustav ^{[6
]}

Casari, Georg ^{[1
]}

Brummelkamp, Thijn R. ^{[2
,3
]}

Superti-Furga, Giulio ^{[2
]}

Nijman, Sebastian M. B. ^{[2
]}

机构：

[1] Haplogen GmbH, Vienna, Austria

[2] Austrian Acad Sci CeMM, Res Ctr Mol Med, Vienna, Austria

[3] Netherlands Canc Inst, Amsterdam, Netherlands

[4] Med Univ Vienna, Lab Infect Biol, Dept Med 1, Vienna, Austria

[5] Med Univ Vienna, Inst Immunol, Vienna, Austria

[6] Univ Vienna, Max F Perutz Labs, Vienna, Austria

来源：

NATURE METHODS | 2013年 / 10卷 / 10期

基金：

欧洲研究理事会; 奥地利科学基金会;

关键词：

EMBRYONIC STEM-CELLS; INACTIVATING MUTATIONS; REQUIRES; IDENTIFICATION; TRANSPORTER; DISRUPTION; DERIVATION; RECEPTORS; CARCINOMA; CASPASE-8;

D O I：

10.1038/nmeth.2609

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Knockout collections are invaluable tools for studying model organisms such as yeast. However, there are no largescale knockout collections of human cells. Using gene-trap mutagenesis in near-haploid human cells, we established a platform to generate and isolate individual 'gene-trapped cells' and used it to prepare a collection of human cell lines carrying single gene-trap insertions. In most cases, the insertion can be reversed. This growing library covers 3,396 genes, one-third of the expressed genome, is DNA-barcoded and allows systematic screens for a wide variety of cellular phenotypes. We examined cellular responses to TNF-alpha, TGF-beta, IFN-gamma and TNF-related apoptosis-inducing ligand (TRAIL), to illustrate the value of this unique collection of isogenic human cell lines.

引用

页码：965 / +

页数：9

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