Vascular endothelial cell adherens junction assembly and morphogenesis induced by sphingosine-1-phosphate

被引：886

作者：

Lee, MJ ^{[1
]}

Thangada, S ^{[1
]}

Claffey, KP ^{[1
]}

Ancellin, N ^{[1
]}

Liu, CH ^{[1
]}

Kluk, M ^{[1
]}

Volpi, M ^{[1
]}

Sha'afi, RI ^{[1
]}

Hla, T ^{[1
]}

机构：

[1] Univ Connecticut, Ctr Hlth, Dept Physiol, Ctr Vasc Biol, Farmington, CT 06030 USA

来源：

CELL | 1999年 / 99卷 / 03期

关键词：

D O I：

10.1016/S0092-8674(00)81661-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Vascular endothelial cells undergo morphogenesis into capillary networks in response to angiogenic factors. We show here that sphingosine-1-phosphate (SPP), a platelet-derived bioactive lipid, activates the EDG-1 and -3 subtypes of G protein-coupled receptors on endothelial cells to regulate angiogenesis. SPP induces the G(i)/mitogen-activated protein kinase/cell survival pathway and the smalt GTPase Rho- and Rac-coupled adherens junction assembly. Both EDG-1- and EDG-3-regulated signaling pathways are required for endothelial cell morphogenesis into capillary-like networks. Indeed, SPP synergized with polypeptide angiogenic growth factors in the formation of mature neovessels in vivo. These data define SPP as a novel regulator of angiogenesis.

引用

页码：301 / 312

页数：12

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