Mck1, a member of the glycogen synthase kinase 3 family of protein kinases, is a negative regulator of pyruvate kinase in the yeast Saccharomyces cerevisiae

被引：26

作者：

Brazill, DT ^{[1
]}

Thorner, J ^{[1
]}

Martin, GS ^{[1
]}

机构：

[1] UNIV CALIF BERKELEY,DEPT MOL & CELL BIOL,DIV BIOCHEM & MOL BIOL,BERKELEY,CA 94720

来源：

JOURNAL OF BACTERIOLOGY | 1997年 / 179卷 / 13期

关键词：

D O I：

10.1128/jb.179.13.4415-4418.1997

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

An interaction between the Saccharomyces cerevisiae protein kinase Mck1 and pyruvate kinase (Pyk1) was detected by using the two-hybrid method. Purified Mck1 was able to phosphorylate purified Pyk1 on Ser in vitro. Pyruvate kinase activity was elevated in mck1 Delta cells. Several of the phenotypes of mck1 Delta mutants are similar to those observed in cells overexpressing PYRI. Co-overexpression of MCK1 suppressed all of the phenotypes associated with PYK1 overexpression. These results indicate that Mck1 negatively regulates pyruvate kinase activity, possibly by direct phosphorylation.

引用

页码：4415 / 4418

页数：4

共 24 条

[1]

Bartel PL., 1993, Cellular interactions in development: A practical approach

[2] A POSITIVE SELECTION FOR MUTANTS LACKING OROTIDINE-5'-PHOSPHATE DECARBOXYLASE ACTIVITY IN YEAST - 5-FLUORO-OROTIC ACID RESISTANCE [J].

BOEKE, JD ;

LACROUTE, F ;

FINK, GR .

MOLECULAR & GENERAL GENETICS, 1984, 197 (02) :345-346

[3] DESIGN OF GLYCOLYSIS [J].

BOITEUX, A ;

HESS, B .

PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY OF LONDON SERIES B-BIOLOGICAL SCIENCES, 1981, 293 (1063) :5-22

[4]

BRAZILL D, 1995, THESIS U CALIFORNIA

[5]

BURKE RL, 1983, J BIOL CHEM, V258, P2193

[6] NOVEL YEAST PROTEIN-KINASE (YPK1 GENE-PRODUCT) IS A 40-KILODALTON PHOSPHOTYROSYL PROTEIN ASSOCIATED WITH PROTEIN-TYROSINE KINASE-ACTIVITY [J].

DAILEY, D ;

SCHIEVEN, GL ;

LIM, MY ;

MARQUARDT, H ;

GILMORE, T ;

THORNER, J ;

MARTIN, GS .

MOLECULAR AND CELLULAR BIOLOGY, 1990, 10 (12) :6244-6256

[7] THE RETINOBLASTOMA PROTEIN ASSOCIATES WITH THE PROTEIN PHOSPHATASE TYPE-1 CATALYTIC SUBUNIT [J].

DURFEE, T ;

BECHERER, K ;

CHEN, PL ;

YEH, SH ;

YANG, YZ ;

KILBURN, AE ;

LEE, WH ;

ELLEDGE, SJ .

GENES & DEVELOPMENT, 1993, 7 (04) :555-569

[8]

Fraenkel D. G., 1982, MOL BIOL YEAST SACCH, P1

[9]

HARPER JW, 1993, CELL, V75, P805

[10] OVEREXPRESSION OF THE YEAST MCK1 PROTEIN-KINASE SUPPRESSES CONDITIONAL MUTATIONS IN CENTROMERE-BINDING PROTEIN GENES CBF2 AND CBF5 [J].

JIANG, WD ;

LIM, MY ;

YOON, HJ ;

THORNER, J ;

MARTIN, GS ;

CARBON, J .

MOLECULAR & GENERAL GENETICS, 1995, 246 (03) :360-366

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