Identification of a ubiquitin-protein ligase subunit within the CCR4-NOT transcription repressor complex

被引：172

作者：

Albert, TK

Hanzawa, H

Legtenberg, YIA

de Ruwe, MJ

van den Heuvel, FAJ

Collart, MA

Boelens, R

Timmers, HTM ^{[1
]}

机构：

[1] Univ Med Ctr, Physiol Chem Lab, NL-3508 AB Utrecht, Netherlands

[2] Univ Utrecht, Bijvoet Ctr Biomol Res, NL-3584 CH Utrecht, Netherlands

[3] Univ Geneva, CMU, Dept Biochem Med, CH-1211 Geneva, Switzerland

来源：

EMBO JOURNAL | 2002年 / 21卷 / 03期

关键词：

CCR4-NOT complex; RING finger; TFIID; transcription regulation; ubiquitin-protein ligase;

D O I：

10.1093/emboj/21.3.355

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The RING finger protein CNOT4 is a component of the CCR4-NOT complex. This complex is implicated in repression of RNA polymerase II transcription. Here we demonstrate that CNOT4 functions as a ubiquitin-protein ligase (E3). We show that the unique C4C4 RING domain of CNOT4 interacts with a subset of ubiquitin-conjugating enzymes (E2s). Using NMR spectroscopy, we detail the interaction of CNOT4 with UbcH5B and characterize RING residues that are critical for this interaction. CNOT4 acts as a potent E3 ligase in vitro. Mutations that destabilize the E2-E3 interface abolish this activity. Based on these results, we present a model of how E3 ligase function within the CCR4-NOT complex relates to transcriptional regulation.

引用

页码：355 / 364

页数：10

共 40 条

[1] Isolation and characterization of human orthologs of yeast CCR4-NOT complex subunits [J].