Efficient generation of recombinant adenoviral vectors by Cre-lox recombination in vitro

被引：101

作者：

Aoki, K

Barker, C

Danthinne, X

Imperiale, MJ

Nabel, GJ

机构：

[1] Univ Michigan, Med Ctr, Howard Hughes Med Inst, Dept Internal Med & Biol Chem, Ann Arbor, MI 48109 USA

[2] Univ Michigan, Med Ctr, Dept Microbiol & Immunol, Ann Arbor, MI USA

[3] Univ Michigan, Med Ctr, Ctr Comprehens Canc, Ann Arbor, MI USA

[4] VA Med Ctr, Res Serv 151, Boise, ID USA

来源：

MOLECULAR MEDICINE | 1999年 / 5卷 / 04期

关键词：

D O I：

10.1007/BF03402119

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Background: Although recombinant adenovirus vectors are attractive for use in gene expression studies and therapeutic applications, the construction of these vectors remains relatively time-consuming. We report here a strategy that simplifies the production of adenoviruses using the Cre-loxP system. Materials and Methods: Full-length recombinant adenovirus DNA was generated in vitro by Cre-mediated recombination between loxP sites in a linearized shuttle plasmid containing a transgene and adenovirus genomic DNA. Results: After transfection of Cre-treated DNA into 293 cells, replication-defective viral vectors were rapidly obtained without detectable wild-type virus. Conclusion: This system facilitates the development of recombinant adenoviral vectors for basic and clinical research.

引用

页码：224 / 231

页数：8

共 27 条

[1] STUDIES ON THE PROPERTIES OF P1 SITE-SPECIFIC RECOMBINATION - EVIDENCE FOR TOPOLOGICALLY UNLINKED PRODUCTS FOLLOWING RECOMBINATION [J].