Grafting neural stem cells improved the impaired spatial recognition in ischemic rats

被引：117

作者：

Toda, H

Takahashi, J ^{[1
]}

Iwakami, N

Kimura, T

Hoki, S

Mozumi-Kitamura, K

Ono, S

Hashimoto, N

机构：

[1] Kyoto Univ, Grad Sch Med, Dept Neurosurg, Sakyo Ku, Kyoto 6068507, Japan

[2] Toyama Chem Co Ltd, Res Labs, Toyama 9308508, Japan

来源：

NEUROSCIENCE LETTERS | 2001年 / 316卷 / 01期

关键词：

neural stem cells; ischemia; cell transplantation; differentiation; water maze test; hippocampus;

D O I：

10.1016/S0304-3940(01)02331-X

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

To determine the possible therapeutic potential of neural stem cells (NSCs) introduced into the damaged central nervous system, we grafted adult hippocampus-derived NSCs into the hippocampus of rats with transient global ischemia. Transient four-vessel occlusion yielded 90-95% losses of pyramidal neurons in the hippocampal CA1 region. In this region, 1-3% of the grafted cells survived; and 3-9% of them expressed NeuN, a neuronal marker. Rats with more than 120 NeuN-positive cells showed partial improvement of impaired spatial learning in a water maze test. These results suggest that NSCs grafted in the ischemic brain are able to differentiate into neurons and to improve spatial recognition. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.

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页码：9 / 12

页数：4

相关论文

共 18 条

[1] Transplanted CNS stem cells form functional synapses in vivo [J].

Auerbach, JM ;

Eiden, MV ;

McKay, RDG .

EUROPEAN JOURNAL OF NEUROSCIENCE, 2000, 12 (05) :1696-1704

[2] SURVIVAL AND FUNCTION OF DISSOCIATED RAT DOPAMINE NEURONS GRAFTED AT DIFFERENT DEVELOPMENTAL STAGES OR AFTER BEING CULTURED INVITRO [J].

BRUNDIN, P ;

BARBIN, G ;

STRECKER, RE ;

ISACSON, O ;

PROCHIANTZ, A ;

BJORKLUND, A .

DEVELOPMENTAL BRAIN RESEARCH, 1988, 39 (02) :233-243

[3] SURVIVAL AND DIFFERENTIATION OF ADULT NEURONAL PROGENITOR CELLS TRANSPLANTED TO THE ADULT BRAIN [J].

GAGE, FH ;

COATES, PW ;

PALMER, TD ;

KUHN, HG ;

FISHER, LJ ;

SUHONEN, JO ;

PETERSON, DA ;

SUHR, ST ;

RAY, J .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (25) :11879-11883

[4] Contrasting effects of fetal CA1 and CA3 hippocampal grafts on deficits in spatial learning and working memory induced by global cerebral ischaemia in rats [J].

Hodges, H ;

Sowinski, P ;

Fleming, P ;

Kershaw, TR ;

Sinden, JD ;

Meldrum, BS ;

Gray, JA .

NEUROSCIENCE, 1996, 72 (04) :959-988

[5] NEUROTROPHINS AND BRAIN INSULTS [J].

LINDVALL, O ;

KOKAIA, Z ;

BENGZON, J ;

ELMER, E ;

KOKAIA, M .

TRENDS IN NEUROSCIENCES, 1994, 17 (11) :490-496

[6] ENHANCED DETECTION OF NUCLEUS BASALIS MAGNOCELLULARIS LESION-INDUCED SPATIAL-LEARNING DEFICIT IN RATS BY MODIFICATION OF TRAINING REGIMEN [J].

MANDEL, RJ ;

GAGE, FH ;

THAL, LJ .

BEHAVIOURAL BRAIN RESEARCH, 1989, 31 (03) :221-229

[7] PLACE NAVIGATION IMPAIRED IN RATS WITH HIPPOCAMPAL-LESIONS [J].

MORRIS, RGM ;

GARRUD, P ;

RAWLINS, JNP ;

OKEEFE, J .

NATURE, 1982, 297 (5868) :681-683

[8] COGNITIVE DEFICITS INDUCED BY GLOBAL CEREBRAL-ISCHEMIA - RELATIONSHIP TO BRAIN-DAMAGE AND REVERSAL BY TRANSPLANTS [J].

NUNN, J ;

HODGES, H .

BEHAVIOURAL BRAIN RESEARCH, 1994, 65 (01) :1-31

[9] GLOBAL-ISCHEMIA - HIPPOCAMPAL PATHOLOGY AND SPATIAL DEFICITS IN THE WATER MAZE [J].

NUNN, JA ;

LEPEILLET, E ;

NETTO, CA ;

HODGES, H ;

GRAY, JA ;

MELDRUM, BS .

BEHAVIOURAL BRAIN RESEARCH, 1994, 62 (01) :41-54

[10] The adult rat hippocampus contains primordial neural stem cells [J].

Palmer, TD ;

Takahashi, J ;

Gage, FH .

MOLECULAR AND CELLULAR NEUROSCIENCE, 1997, 8 (06) :389-404