Interleukin-18 activates NF-kappa B in murine T helper type 1 cells

被引：173

作者：

Matsumoto, S ^{[1
]}

TsujiTakayama, K ^{[1
]}

Aizawa, Y ^{[1
]}

Koide, K ^{[1
]}

Takeuchi, M ^{[1
]}

Ohta, T ^{[1
]}

Kurimoto, M ^{[1
]}

机构：

[1] HYASHIBARA BIOCHEM LABS INC,FUJISAKI INST,OKAYAMA 702,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1997年 / 234卷 / 02期

关键词：

D O I：

10.1006/bbrc.1997.6665

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Interleukin-18 (IL-18) activated T helper type 1 (Th1) cells, OVA#4, and induced production of interleukin-2 (IL-2) in costimulation with anti-CD3 antibody. Upon stimulation with IL-18, I kappa B disappeared from cytoplasm and subsequently nuclear factor-kappa B (NF-kappa B) (p65) accumulated in the nucleus. Corresponding with that, DNA binding activity of NF-kappa B (p65 homodimer or p65/p50 heterodimer) was detected in the nucleus. In the transfection experiments, an IL-2 promoter-driven reporter construct showed the similar responsiveness against IL-18 to that of the intrinsic IL-2 gene, and a construct lacking kappa B site failed to respond to IL-18. These results suggest that IL-18 activates NF-kappa B and it is important for enhancement of IL-2 gene expression by Th1 cells stimulated with IL-18. (C) 1997 Academic Press.

引用

页码：454 / 457

页数：4

共 16 条

[1] The NF-kappa B and I kappa B proteins: New discoveries and insights [J].

Baldwin, AS .

ANNUAL REVIEW OF IMMUNOLOGY, 1996, 14 :649-683

[2]

BANKERSFULBRIGH.L, 1996, LIFE SCI, V59, P61

[3]

DiDonato J, 1996, MOL CELL BIOL, V16, P1295

[4] HIGH-EFFICIENCY DNA-MEDIATED TRANSFORMATION OF PRIMATE CELLS [J].

GORMAN, C ;

PADMANABHAN, R ;

HOWARD, BH .

SCIENCE, 1983, 221 (4610) :551-553

[5] Transcriptional regulation of the interleukin-2 gene in normal human peripheral blood T cells - Convergence of costimulatory signals and differences from transformed T cells [J].

Hughes, CCW ;

Pober, JS .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (10) :5369-5377

[6] THE AP-1 SITE AT -150 BP, BUT NOT THE NF-KAPPA-B SITE, IS LIKELY TO REPRESENT THE MAJOR TARGET OF PROTEIN-KINASE-C IN THE INTERLEUKIN-2 PROMOTER [J].

JAIN, J ;

VALGEARCHER, VE ;

SINSKEY, AJ ;

RAO, A .

JOURNAL OF EXPERIMENTAL MEDICINE, 1992, 175 (03) :853-862

[7]

JAIN JN, 1993, J IMMUNOL, V151, P837

[8]

Kohno K, 1997, J IMMUNOL, V158, P1541

[9]

Lederer JA, 1996, J IMMUNOL, V156, P56

[10] IL-1 RECEPTOR AND TCR SIGNALS SYNERGIZE TO ACTIVATE NF-KAPPA-B-MEDIATED GENE-TRANSCRIPTION [J].

MCKEAN, DJ ;

BELL, M ;

HUNTOON, C ;

RASTOGI, S ;

VANNORSTRAND, M ;

PODZORSKI, R ;

NILSON, A ;

PAYA, C .

INTERNATIONAL IMMUNOLOGY, 1995, 7 (01) :9-20

← 1 2 →