A citric acid solution is an optimal test drink in the C-13-urea breath test for the diagnosis of Helicobacter pylori infection

Background-The C-13-urea breath test (C-13-UBT) is a simple, non-invasive and reliable test for the diagnosis of Helicobacter pylori infection. The duration of the test, the timing of breath sampling, and the accuracy of the method vary according to the test meal used. Aim-To identify the optimal test meal or drink for rapid and accurate performance of the C-13-UBT for the detection of H pylori infection. Patients-Eighty patients with dyspeptic symptoms were included. Of these, 48 patients had a positive H pylori status and 32 a negative one according to the results of the rapid urease test, histological examination, and culture. Methods-A C-13-UBT was performed after an overnight fast, on three consecutive days. On each study day a different test meal or drink was given (0.1 N citric acid solution, a standard semiliquid meal, or a semiliquid fatty meal) 10 minutes before giving 75 mg C-13-urea. Breath samples were collected at 0, 15, 30, 45, and 60 minutes, and analysed by isotype ratio mass spectrometry. Results were expressed as delta (delta) and considered as positive for H pylori if the highest delta (peak) was greater than 4.0. Results-The delta peak obtained with the citric acid drink in H pylori positive subjects (24.1 (SEM 1.5)) was significantly higher than that obtained with any of the semiliquid meals (13.3 (SEM 1.1) and 17.1 (SEM 1.0) respectively, p < 0.001). Futhermore, this 6 peak was obtained earlier with the citric acid drink (30 (SEM 2) minutes) than with the other two meals tested (53 (SEM 2) min and 45 (SEM 2) min, p < 0.001). The sensitivity of the C-13-UBT for the diagnosis of H pylori infection was 96-100% with all three test meals. This high sensitivity was, however, obtained from 15 minutes by giving citric acid as the test drink, from 45 minutes by giving a semiliquid fatty meal, and at 60 minutes by giving the semiliquid standard meal. The specificity was 100% for all test meals. Citric acid is inexpensive and palatable to patients. Conclusions-The C-13-UBT procedure with citric acid as the test drink is superior to the previously proposed semiliquid test meals in terms of (CO2)-C-13 recovery, time requirement, and cost. In routine clinical sampling, collection at times 0 and 30 minutes seems to be optimal and gives a high diagnostic accuracy.