Capture of Authentic Embryonic Stem Cells from Rat Blastocysts

被引：623

作者：

Buehr, Mia ^{[2
,3
]}

Meek, Stephen ^{[2
,3
]}

Blair, Kate ^{[1
,4
]}

Yang, Jian ^{[1
,5
]}

Ure, Janice ^{[2
]}

Silva, Jose ^{[1
,4
]}

McLay, Renee ^{[2
]}

Hall, John ^{[1
,4
]}

Ying, Qi-Long ^{[2
]}

Smith, Austin ^{[1
,4
]}

机构：

[1] Univ Cambridge, Wellcome Trust Ctr Stem Cell Res, Cambridge CB2 1QR, England

[2] Univ Edinburgh, Inst Stem Cell Res, Edinburgh EH9 3JQ, Midlothian, Scotland

[3] Univ Edinburgh, Roslin Inst, Roslin EH25 9PS, Midlothian, Scotland

[4] Univ Cambridge, Dept Biochem, Cambridge CB2 1QW, England

[5] Fudan Univ, Shanghai Med Coll, Minist Educ, Key Lab Mol Med, Shanghai 200032, Peoples R China

来源：

CELL | 2008年 / 135卷 / 07期

基金：

英国医学研究理事会; 美国国家卫生研究院; 英国生物技术与生命科学研究理事会;

关键词：

D O I：

10.1016/j.cell.2008.12.007

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Embryonic stem (ES) cells have been available from inbred mice since 1981 but have not been validated for other rodents. Failure to establish ES cells from a range of mammals challenges the identity of cultivated stem cells and our understanding of the pluripotent state. Here we investigated derivation of ES cells from the rat. We applied molecularly defined conditions designed to shield the ground state of authentic pluripotency from inductive differentiation stimuli. Undifferentiated cell lines developed that exhibited diagnostic features of ES cells including colonization of multiple tissues in viable chimeras. Definitive ES cell status was established by transmission of the cell line genome to offspring. Derivation of germline-competent ES cells from the rat paves the way to targeted genetic manipulation in this valuable biomedical model species. Rat ES cells will also provide a refined test-bed for functional evaluation of pluripotent stem cell-derived tissue repair and regeneration.

引用

页码：1287 / 1298

页数：12

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