Subcellular [Ca2+]i gradients during excitation-contraction coupling in newborn rabbit ventricular myocytes

The central role of T-tubule and sarcoplasmic reticulum (SR) diadic junctions in excitation-contraction (EC) coupling in adult (AD) ventricular myocytes suggests that their absence in newborn (NB) cells may manifest as an altered EC coupling phenotype. We used confocal microscopy to compare fluo-3 [Ca2+](i) transients in the subsarcolemmal space and cell center of field-stimulated NE and AD rabbit ventricular myocytes. Peak systolic [Ca2+](i) occurred sooner and was higher in the subsarcolemmal space compared with the cell center in NE myocytes. In AD myocytes, [Ca2+](i) rose and declined with similar profiles at the cell center and subsarcolemmal space. Disabling the SR (10 mu mol/L thapsigargin) slowed the rate of rise and decline of Ca2+ in AD myocytes but did not alter Ca2+ transient kinetics in NE myocytes. In contrast to adults, localized SR Ca2+ release events ("Ca2+ sparks") occurred predominantly at the cell periphery of NE myocytes. Immunolabeling experiments demonstrated overlapping distributions of the Na+-Ca2+ exchanger and ryanodine receptors (RyR2) in AD myocytes. In contrast, RyR2s were spatially separated from the sarcolemma in NE myocytes. Confocal sarcolemmal imaging of di-8-ANEPPS-treated myocytes confirmed an extensive T-tubule network in AD cells, and that T-tubules are absent in NE myocytes. A mathematical model of subcellular Ca2+ dynamics predicts that Ca2+ flux via the Na+-Ca2+ exchanger during an action potential can account for the subsarcolemmal Ca2+ gradients in NE myocytes. Spatial separation of sarcolemmal Ca2+ entry from SR Ca2+ release channels may minimize the role of SR Ca2+ release during normal EC coupling in NE ventricular myocytes.