Targeted Heritable Mutation and Gene Conversion by Cas9-CRISPR in Caenorhabditis elegans

被引：82

作者：

Katic, Iskra ^{[1
]}

Grosshans, Helge ^{[1
]}

机构：

[1] Friedrich Miescher Inst Biomed Res, CH-4058 Basel, Switzerland

来源：

GENETICS | 2013年 / 195卷 / 03期

基金：

欧洲研究理事会; 瑞士国家科学基金会; 美国国家卫生研究院;

关键词：

genome editing; CRISPR; Cas9; homologous recombination; gene conversion; Caenorhabditis elegans; ZINC-FINGER TARGETER; CRISPR-CAS SYSTEMS; C; ELEGANS; GENOME; DROSOPHILA; DAF-2; TRANSPOSON; RECEPTOR; BREAKS; ZIFIT;

D O I：

10.1534/genetics.113.155754

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

We have achieved targeted heritable genome modification in Caenorhabditis elegans by injecting mRNA of the nuclease Cas9 and Cas9 guide RNAs. This system rapidly creates precise genomic changes, including knockouts and transgene-instructed gene conversion.

引用

收藏

页码：1173 / 1176

页数：4

相关论文

共 35 条

[1] Mobilization of a Drosophila transposon in the Caenorhabditis elegans germ line [J].

Bessereau, JL ;

Wright, A ;

Williams, DC ;

Schuske, K ;

Davis, MW ;

Jorgensen, EM .

NATURE, 2001, 413 (6851) :70-74

[2] Transgene-Free Genome Editing in Caenorhabditis elegans Using CRISPR-Cas [J].

Chiu, Hui ;

Schwartz, Hillel T. ;

Antoshechkin, Igor ;

Sternberg, Paul W. .

GENETICS, 2013, 195 (03) :1167-1171

[3] Heritable Gene Knockout in Caenorhabditis elegans by Direct Injection of Cas9-sgRNA Ribonucleoproteins [J].

Cho, Seung Woo ;

Lee, Jihyun ;

Carroll, Dana ;

Kim, Jin-Soo ;

Lee, Junho .

GENETICS, 2013, 195 (03) :1177-+

[4] Multiplex Genome Engineering Using CRISPR/Cas Systems [J].

Cong, Le ;

Ran, F. Ann ;

Cox, David ;

Lin, Shuailiang ;

Barretto, Robert ;

Habib, Naomi ;

Hsu, Patrick D. ;

Wu, Xuebing ;

Jiang, Wenyan ;

Marraffini, Luciano A. ;

Zhang, Feng .

SCIENCE, 2013, 339 (6121) :819-823

[5] Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas systems [J].

DiCarlo, James E. ;

Norville, Julie E. ;

Mali, Prashant ;

Rios, Xavier ;

Aach, John ;

Church, George M. .

NUCLEIC ACIDS RESEARCH, 2013, 41 (07) :4336-4343

[6] GENETIC AND MOLECULAR ANALYSIS OF A CAENORHABDITIS-ELEGANS BETA-TUBULIN THAT CONVEYS BENZIMIDAZOLE SENSITIVITY [J].

DRISCOLL, M ;

DEAN, E ;

REILLY, E ;

BERGHOLZ, E ;

CHALFIE, M .

JOURNAL OF CELL BIOLOGY, 1989, 109 (06) :2993-3003

[7] Heritable genome editing in C. elegans via a CRISPR-Cas9 system [J].

Friedland, Ari E. ;

Tzur, Yonatan B. ;

Esvelt, Kevin M. ;

Colaiacovo, Monica P. ;

Church, George M. ;

Calarco, John A. .

NATURE METHODS, 2013, 10 (08) :741-+

[8] Single-copy insertion of transgenes in Caenorhabditis elegans [J].

Frokjaer-Jensen, Christian ;

Davis, M. Wayne ;

Hopkins, Christopher E. ;

Newman, Blake J. ;

Thummel, Jason M. ;

Olesen, Soren-Peter ;

Grunnet, Morten ;

Jorgensen, Erik M. .

NATURE GENETICS, 2008, 40 (11) :1375-1383

[9] Improved Mos1-mediated transgenesis in C. elegans [J].

Frokjaer-Jensen, Christian ;

Davis, M. Wayne ;

Ailion, Michael ;

Jorgensen, Erik M. .

NATURE METHODS, 2012, 9 (02) :117-118

[10]

Frokjær-Jensen C, 2010, NAT METHODS, V7, P451, DOI [10.1038/NMETH.1454, 10.1038/nmeth.1454]

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