Metarhodopsin-II stabilization by crosslinked Gtα C-terminal peptides and implications for the mechanism of GPCR-G protein coupling

被引：6

作者：

Angel, Thomas E.

Kraft, Paul C.

Dratz, Edward A. ^{[1
]}

机构：

[1] Montana State Univ, Dept Chem & Biochem, Bozeman, MT 59717 USA

[2] Montana State Univ, Dept Microbiol, Bozeman, MT 59717 USA

来源：

VISION RESEARCH | 2006年 / 46卷 / 27期

关键词：

metarhodopsin-II; mass spectrometry; chemical crosslinking; transducin;

D O I：

10.1016/j.visres.2006.08.015

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Metarhodopsin-II is the light-excited form of rhodopsin that triggers G protein function. Metarhodopsin-II is stabilized when the N-terminus of the carboxyl (340-350) tail peptide of the a-subunit of transducin (Gt alpha) is crosslinked to rhodopsin cysteine 140 or the 340-350 peptide C-terminus of Gta is crosslinked to rhodopsin cysteine 316. When the N-terminus of the peptide is coupled to C316 the MI <-> MII equilibrium is not affected. The evidence suggests that the N-terminus of the 340-350 region of Gta is located near C140 when transducin stabilizes metarhodopsin-II and alternative explanations are suggested for the effectiveness of the 340-350 Gta tail peptide when bound to C316. (c) 2006 Elsevier Ltd. All rights reserved.

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收藏

页码：4547 / 4555

页数：9

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